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Sera taken from 449 cows which aborted in years 1991-1994 have been assessed by the complement fixation test for the presence of Q-fever antibodies. It was found that 10.7% of the animals had antibodies against Coxiella burnetii II antigen. Moreover, in the examinations repeated three times on 150 cows coming from a farm in which several cases of abortions were observed, about 15% of seroreagents...
The objective of the study was to evaluate the effectiveness of various methods of RNA extraction from feces of diarrhoeic piglets to diagnose rotaviral infections by the polymerase chain reaction (PCR). The most effective among the four methods of extraction appeared to be the extraction of RNA with quanidine thiocyanate followed by purification on glass powder. This method also enabled an effective...
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