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In order to identify trichothecenes chemotypes produced by Fusarium graminearum species complex (FGSC) isolated from barley, 68 barley samples were collected from markets in Kermanshah and Hamedan provinces, Iran. Thirty-one Fusarium isolates were obtained from grains and morphologically classified into three species FGSC (14), F. equiseti (9), and F. proliferatum (8). The identification of the members...
In recent years, oil spill accidents have become increasingly frequent due to the development of marine transportation and massive oil exploitation. At present, satellite remote sensing is the principal method used to monitor oil spills. Extracting the locations and extent of oil spill spots accurately in remote sensing images reaps significant benefits in terms of risk assessment and clean-up work...
Compared to angiosperms, conifers represent more complex genomes with larger giga-genome size. To detect large-scale single nucleotide polymorphisms (SNPs), whole genome sequencing of a conifer population is still unaffordable. In this work, we report the use of DNA specific-locus amplified fragment sequencing (SLAF-seq) for large-scale SNP detection in Chinese fir (Cunninghamia lanceolata (Lamb.)...
A Polish isolate of Soil-borne wheat mosaic virus (SBWMV-Pol1) was characterized by limited pathogenicity and a low concentration of virus particles in infected plant tissues. The aim of this research was to consider the possibility of seed-transmission dissemination of the virus. Seeds of winter wheat cv. Muszelka served as material for the studies. Two methods were involved in the diagnostics of...
Among the numerous protocols that describe the extraction of DNA, those relating to the isolation of DNA from infected plants, are rare. This study describes a rapid and reliable method of extracting a high quality and quantity of DNA from rhododendron leaves artificially infected with Phytophthora cactorum, P. cambivora, P. cinnamomi, P. citrophthora, and P. plurivora. The use of the modified Doyle...
Xanthomonas hortorum pv. pelargonii is the causal agent of bacterial blight of geranium. A specific and rapid real-time PCR assay for detecting the bacterium in plant was developed in this study. We compared sensitivity of conventional and real-time PCR for detection of the pathogen in inoculated plants. For application to disease management programs, PCR amplification must be able to detect latent...
Thrips from the genus Frankliniella (Thysanoptera, Thripidae) are phytophagous on crops and wild plants. Some of them cause slight economic damage, however, others including F. occidentalis and F. intonsa are responsible for considerable losses in crop production. Moreover, they constitute a double threat for host plants by not only feeding on them but also vectoring viruses, some of which are on...
The paper describes a microbiological method for the detection of antibacterial substances in feedingstuffs. The method allowed detection of the main antibiotic groups, including tetracyclines. In 2013-2014, a total of 171 feed samples were analysed to determine antibacterial substances. Among the analysed samples 84 (49.1%) were suspected to contain tetracyclines. Out of the 84 feeds analysed using...
Iris belongs to the Iridaceae family. It is one of the most important pharmaceutical and ornamental plants in the world. To assess the potyvirus incidence in natural resources of iris plants in Iran, Antigen Coated-Plate ELISA (ACP-ELISA) was performed on 490 symptomatic rhizomatous iris leaf samples, which detected the potyvirus in 36.7% of the samples. Genomic 3’ end of one mechanically non-transmitted...
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