Journal of Plant Protection Research
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Journal of Plant Protection Research > 1997 > 37 > 1-2 > 18-27
The isometric virus isolated from several lettuce plantations appeared to be dandelion yellow mosaic sequivirus. Despite of some differences it seemed to be closely related to Dutch Ls2 isolate. Identification was based on the host range, physico-chemical properties, electron microscopy and serology. The differences in virus susceptibility were found between 12 cultivars mechanically inoculated. None...
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The technology used to amplify and isolate the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene of Chironomus tentans is described i.e. the Polymerase Chain Reactioin and the agarose gel electrophoresis of this reaction product, as well as the blunting reaction of the product and its isolation.
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In the years 1993-1995 experiments on integrated pest management (IPM) were conducted. A three year experiment with winter wheat was performed to evaluate the effect of chemical control of diseases on their occurrence and grain yield, at various conditions of N fertilisation. The economical profit was analysed.
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In the leaves of four apple cultivars with a different degree of susceptibility to the apple rust mite - Aculus schlechtendali (Nal.) the content of some plant components was estimated. It has been found that a long-term feeding of the apple rust mite induces changes in the content of reducing sugars. These changes were significantly varying depending on the apple cultivar.
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Journal of Plant Protection Research > 1997 > 37 > 1-2 > 28-44
Diagnostic studies were performed on 4 viruses isolated from several lettuce plantations in the central region of Poland. Identification was based on the host range, including tests plants and different lettuce cultivars, on the characteristics of virus transmissibility, as well as on their physico-chemical properties, electron microscopy and serology.
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Different mechanisms of regulation regarding the Open Reading Frames (ORFs) have been discussed to have a broader perspective that is necessary to evaluate the role of the ORFs of the I-18 C gene. This consideration includes the ORF II of the I-18 C gene which is the object of the presented research.
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Six fungal species, Aspergillus flavus Link ex Fr., Beauveria bassiana (Bals.) Vuill., Acremonium sensu Gams, Fusarium sp. Link ex Fr., Paecilomyces farinosus (Holm. ex Gray) Brown et Smith as well a Paecilomyces fumosoroseus (Wize) Brown et Smith, have been isolated as result of studies on entomopathogenic fungi of the satin moth Stilpnotia salicis. The epizootic induced by the fungus B. bassiana...
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Journal of Plant Protection Research > 1997 > 37 > 1-2 > 52-58
The simple method of nucleic acids extraction, based on guanidine thiocyanate extraction buffer, was sufficient for obtaining a good templates for RT-PCR. The RT-PCR reactions were performer from the start to the end (both the reverse transcription and the HLVd-cDNA amplification) in the same reaction mixture and in the very small volume of reaction (10 μI). Both pairs of primers designed by authors...
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Journal of Plant Protection Research > 1997 > 37 > 1-2 > 85-93
In 1995, observations were conducted on the intensity of the Colorado beetle on potato varieties and strains. The studies showed variation in this pest occurrence on different varieties and strains.
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The content and composition of glucosinolates in the leaves of some double low and traditional varieties of oilseed rape (Brassica napus L.), at three different stages of development, was investigated. Double low rape varieties showed lower, general content of glucosinolates of which indolyl glucosinolates were the most abundant, specially at the initial stage of plant development.
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Journal of Plant Protection Research > 1997 > 37 > 1-2 > 104-108
The content and composition of glucosinolates in the leaves of some double low and traditional varieties of oilseed rape (Brassica napus L.), at three different stages of development, was investigated. Double low rape varieties showed lower, general content of glucosinolates of which indolyl glucosinolates were the most abundant, specially at the initial stage of plant development.
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The technology for the preparation of the template in the research aimed to isolate the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene is presented i.e. the linearisation of the template by Bam H I digestion, the purification of the template and the estimation of the template concentration.
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The above mentioned technology in a case of the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene is described i.e. the primers design, purification and phosphorylation of the oligonucleotide primers.
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Diagnostic studies were performed on 4 viruses isolated from several lettuce plantations in the central region of Poland. Identification was based on the host range, including tests plants and different lettuce cultivars, on the characteristics of virus transmissibility, as well as on their physico-chemical properties, electron microscopy and serology.
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The hop (Humulus lupulus L.) is one of the most infected plants by viruses. Preparation of apical meristems in combination with heat therapy was successfuly used for obtaining of virus-free hop plants in various countries in the world. In our experiments we prepared the meristems both before and after the heat therapy. Hop plants regenerated from such meristems were free not only from the thermolabile...
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Secondary plant substances - glucosinolates and saponins reduced seed germination and seedling growth of lettuce. Saponins showed greater inhibitory effect on both germination and growth of lettuce seedlings than glucosinolates. The effect depended on the concentration of these compounds in the ground. Tested compounds caused abbreviation of roots, browning their tips and prevented root hair development.
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Most of the surveyed lettuce field plantations in central region of Poland were shown to be virus infected. Percentage of infected plants was however low. Five viruses have been isolated and identified, namely lettuce mosaic potyvirus (LMV), dandelion yellow mosaic sequivirus (DYMV), lettuce big vein varicosavirus (LBVV), tomato mosaic tobamovirus (ToMV) and cucumber mosaic cucumovirus (CMV). Most...
article
The simple method of nucleic acids extraction, based on guanidine thiocyanate extraction buffer, was sufficient for obtaining a good templates for RT-PCR. The RT-PCR reactions were performer from the start to the end (both the reverse transcription and the HLVd-cDNA amplification) in the same reaction mixture and in the very small volume of reaction (10 μI). Both pairs of primers designed by authors...
