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Recent studies uncovered an important role of aquaporins in cell migration, and migration-associated cell function such as angiogenesis, wound healing, and neutrophil motility. Here we provide evidence that aquaporin expression is involved in MCF-7 human mammary carcinoma cell migration. Reverse transcription-polymerase chain reaction, immunoblot and immunofluorescence analysis demonstrated two populations...
Recent studies have demonstrated that aquaporin (AQP) expression facilitates cell migration and promotes angiogenesis and neutrophil motility. Migration of tumor cells is a crucial step in tumor invasion and metastasis. In the present study, we investigated the expression of AQP in human W489 colon cancer cells and characterized its function in cell migration. Two populations of W489 cell clones with...
Laser scanning confocal technology was used to study relationship between apoptosis and changes of Ca2+ induced by CSA (Capparis spinosa L. total alkaloid, CSA) on human heptocarcinoma cell HepG-2. Killing effects of CSA on human heptocarcinoma cell HepG-2 was measured by MTT method, while morphological observation on HepG-2 cells was completed by fluorescence microscope. Apoptosis of HepG-2 cells...
Tumour invasion and metastatic dissemination depends on cell motility, a process that may be studied and quantitatively evaluated by analysing multidimensional digital images. Current methods allow to keep track of the paths followed by specific objects and perform statistical analysis on the recorded data. By adapting a set of tools, independently developed for studying cell movement, to work with...
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