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High-throughput applications on time-lapse microscopy allows us to follow the in vitro temporal and spatial evolution of cell populations; analysis on those kind of data will reveal cell motion parameters such as average speed, persistence and directionality, important informations for many research and therapeutic applications such as drug development or wound healing. The large quantity of frames...
The neurotransmitter acetylcholine (Ach) controls both excitatory and inhibitory synaptic transmission in the striatum. Here, we investigated the involvement of the endocannabinoid system in Ach-mediated inhibition of striatal GABA transmission, and the potential role of transient receptor potential vanilloid 1 (TRPV1) channels in the control of Ach-endocannabinoid coupling. We found that inhibition...
Tumour invasion and metastatic dissemination depends on cell motility, a process that may be studied and quantitatively evaluated by analysing multidimensional digital images. Current methods allow to keep track of the paths followed by specific objects and perform statistical analysis on the recorded data. By adapting a set of tools, independently developed for studying cell movement, to work with...
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