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Viral load quantification is a critical need for HIV management worldwide. However, the diagnostic technologies currently available are too limited by their size and expense to reach many remote and resource-limited populations. Toward the development of techniques which can be leveraged for point-of-care assays, we have investigated affinity capture of whole viruses using magnetic microparticles...
Thermophiles are microorganisms that inhabit at extremely high temperature environment. Thermoenzymes from thermophilic microorganisms are heat-stable, able to withstand denaturants of extremely acidic and alkaline conditions, and thus, have a considerable potential for many industrial applications. The aim of this study was to isolate and characterize amylolytic thermophiles. Two isolates of amylase...
Porcine transmissble gastroenteritis (TGE) is a highly contagious enteric disease of swine caused by transmissible gastroenteritis virus (TGEV). The disease is especislly severe in piglets under two weeks of age, characterized by vomiting, diarrhea and nearly 100% mortality. In this study, the complete genome sequence is available from SC-Y strain isolated in Sichuan province. Sixteen overlapping...
Salmonella pullorum was induced using liquid lysogeny broth medium at 4°C into viable but non-culturable (VBNC) state. The activated VBNC genes were screened by mRNA differential display. VBNC bacteria transformed to the spherical-rods or spherical shape and aggregated. Sequence analysis showed two VBNC differential fragments respectively belonged to partial sequences of ATP-dependent RNA unwindase...
The suppression effect of Bacillus amyloliquefacien Ba33 against TMV and PVY by biological detect through necrotic lesions host and RNA detect through RT-PCR was studied. The result showed that the suppression ratio of necrotic lesions against TMV and PVY was 95.25% and 99.22% respectively. And Ba33 could suppression RNA replication of TMV and PVY in Nicotiana tabacum var. NC89 leaves from 24 to 84h...
In this study, the antigenic site B/C of S gene of transmissible gastroenteritis virus (TGEV) was cloned into prokaryotic expression vector pET-28a and the recombinant plasmid was transferred into E.coli BL21 (DE3). The fusion protein was expressed after induction with IPTG. The SDS-PAGE purified protein was used as immunogen. Six-week-old BALB/c mice were immunized three times with recombinant protein...
The DDK syndrome is known as the polar-lethal embryonic death that occurs at the morula-blastocyst stage when female mice of the DDK strain are mated with males from many other inbred strains (alien males). Embryonic death is interpreted to be caused by incompatibility between a DDK oocyte factor and the product from male pronucleus, both of which map to the Om locus on mouse chromosome 11. We compared...
To better understand the osmo-adaption mechanism in the Torulopsis versatilis, we investigated the salt-tolerant gene HOG1 from the wild-type T. versatilis. Genome of T yeast was used for PCR reaction to amplify the HOG1 gene. The PCR productions were sent into sequencing vector pUC19 to achieve the plasmid pUC19-HOG1. The engineered strain W303-YEp195-T and the control strain W303-YEp195 were constructed,...
Small regulatory RNAs are highly abundant noncoding RNAs (ncRNA) found in bacterial genomes. These small regulatory ncRNAs (sRNAs) can regulate the synthesis of proteins by mediating mRNA transcription, translation and stability. Furthermore, they also control the activity of specific proteins by binding to them. In this study, we present a general computational approach for identifying the distinct...
A TuMV-NIb gene fragment was amplified from the total RNA of Mustard leaves infected with TuMV-GZ strain by RT-PCR (Reverse Transcription Polymerase Chain Reaction). This fragment was cloned into plant binary vector pBI121 in the antisense orientation and transformed into Mustard (Brassica juncea L. Linshicaoyaozi) by Agro bacterium tumefaciens mediated method. 11transgenic T0 plants were obtained...
The effects and mechanisms were studied on killing of Escherichia coli, a Gram negative bacterium by exogenous hydroxyl radical. The results showed that, exogenous OH· was efficient on killing of E. coli cells. Over 99% of them was killed in 0.5 mg/L OH· solution. And exogenous OH· could damage biomolecules of E. coli. The concentrations and integrities of DNA, RNA and intracellular protein decreased...
Computational ribonucleomics of heavy metal stress tolerant Bacillus sp. strain SJ-101 16SrRNA gene has led to the identification of a novel small non-coding RNA (sRNA) and promoter-like sequences within 3' region of the gene. Using RNAz web server, a 123 bp long functional sRNA with significant thermodynamic stability (Energy contribution: -25.2) and evolutionary conservation (structure conservation...
The primers and probes were designed and synthesized according to the conserved VP1 sequences of porcine Sapovirus (SaV), and a TaqMan fluorescence quantitative RT-PCR assay were developed by optimizing the reaction conditions. Results showed that the fluorescence quantitative RT-PCR assay could detect 16.1 copies·µL−1 of plasmid DNA, while the sensitivity of the routine RT-PCR was 1.61 × 103 copies·µL...
This work is focused on modelling HIV mutation and drug resistance. We develop a technique to study viral dynamics in a system of m viral strains including mutation between the different strains. Stability analysis of the nonlinear dynamics involving the free virus and its host cell is provided. For the class of model considered, with ??low?? mutation rate, we analytically determine all possible equilibria,...
Agaricus bisporus is a delicious, nutritious mushroom with medical function. Cross breeding is limited due to its taxonomic contradiction. We analyzed rDNA ITS sequences of two high quality Agaricus bisporus strains (No.Tb07, As2796). Tb07 strain was selected from foreign mushroom by CSUFT under protection. As2796 strain was a high quality local strain in nature in China. By sequencing the ITS regions...
Xylanase (E.C 3.2.1.8) is a widespread group of enzyme which can catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in xylan. Xylanase has a wide application in industrial processes. In this study, the total RNA was isolated, the mature xylanase gene was obtained from Aspergillus niger C3486 by RT-PCR. To achieve secretive expression, pET32a (+) was used as expression vector, and the cloned...
We fabricated laser diodes containing highly stacked InAs quantum dots (QDs) using the strain-compensation technique, which showed laser emission at 1.7 mum above the threshold current of 400 mA in pulsed mode.
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