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The practical application of dielectrometry method to identify the pharmaceutical ingredients was held. The proposed waveguide-differential dielectrometer is used for the complex permittivity determination of high loss liquid such as water and water solutions in the millimeter wave range. This method allows a real-time determination of pharmaceutical ingredient content in water solution by measuring...
We have been developing a nondestructive quantitative inspection technique for pharmaceutical tablets. We constructed a compact optical system to stabilize signal intensity, in which a galvano mirror was applied for adjusting incident angle of seeding beam and a 2D-CMOS sensor was used for signal detection. We evaluated its performance and confirmed that 0.5wt% of DL-histidine in L-histidine could...
Standard pharmaceutical processes involving cell lines are generally monitored by expensive and time consuming laboratory testing by direct sampling of the culture media to evaluate cell state and quality. Conversely to standard off-line measurements, in this work we propose a novel miniaturized biosensor array to detect glucose and lactate in-situ and on-line. The electrochemical biosensors are accomplished...
A biosensor for the detection of ascorbic acid is developed by preparing a carbon paste electrode (CPE) electrochemically deposited with Prussian blue (PB) and DNA adsorbed on its surface. Cyclic voltammetry (CV) was used to study the electrochemical properties of the biosensor. The successful electro-deposition of PB was confirmed using Raman Spectroscopy. From the results it was concluded that PB...
In order to enhance cell and spore concertration of Bacillus Licheniformis SH003, the optimum fermentation conditions of Bacillus Licheniformis SH003 were studied by single-factor test in shake flasks conditions. The results indicated that culture conditions were: agar volume 40mL, inoculum concentration 10%, initial pH 6.5, growth temperature 35°C, fermentation time 36h and shaker rotating speed...
Routine measurements of blood biochemistry in fish are important in research. Anesthetic is generally used before blood sampling to prevent injury to the fish; however anesthetic may also act as a stressor and influence the blood parameter measurements. The effects of tricaine methanesulfonate (MS-222) on the blood biochemistry of endangered Chinese sturgeon (Acipenser sinensis) were studied to better...
Pyrofomes demidoffii is a new recorded species of the new recorded genus in the China. To study the high yield and economical medium productive conditions, this experiment selected and used single factors such as carbon source, nitrogen source, C/N rations, rational speed, temperature, pH value, bottled volume and inoculated bulk and multifactor experiment design with submerged fermentation mycelia...
Alkaline (NaOH) hydrolysis and aqueous extraction method were comparatively studied in polysaccharides extraction from mycelia of Grifola frondosa obtained with submerged-culture. The results show that the polysaccharide content and the total extract rate from alkaline extraction method were obviously higher than those from the aqueous extraction method. The conditions of alkaline extraction were...
The amylomaltase gene of the Streptomyces ST66 was cloned. The open reading frame of this gene consisted of 2,136 nucleotides and encoded a polypeptide that was711 amino acids. The amylomaltase gene was overexpressed in E. coli. The detailed action profile of crude enzyme from E.coli Top 10F'/pTrc-CKS- MalQ on amylose was analyzed and tested whether this crude enzyme could catalyze cyclization of...
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