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Ligand-receptor binding site model is used to elucidate the binding affinity between ligands and receptors, with transistor-based sensors. AlGaN/GaN high electron mobility transistors (HEMTs) were immobilised with antibodies and human immunodeficiency virus type 1 reverse transcriptase enzymes to detect peptides and human immunodeficiency virus drugs, respectively. The signals generated by the sensors...
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a secreted protein that regulates the number of cell surface low‐density lipoprotein receptors (LDLRs) and the levels of low‐density lipoprotein cholesterol in plasma. Intact cells have not previously been used to determine the characteristics of binding of PCSK9 to LDLR. Using PCSK9 iodinated by the tyramine cellobiose (TC) method ([125I]TC‐PCSK9),...
Surface plasmon resonance (SPR) is a widely used label-free detection technique that has many applications in drug discovery, pharmacokinetics, systems biology and food science. The SPR technique measures the dynamics of a biomolecular interaction at a surface, yielding kinetic association and dissociation constants. Present SPR systems measure the step response of the interaction in time domain hence...
A central challenge of modern biology is the need to understand how the interactions of protein machines affect cellular physiology and the pathology. Optical imaging and spectroscopy afford unprecedented opportunities in studying these dynamical processes in vivo. Time-resolved fluorescence resonance energy transfer microscopy has been developed to study mechanotransduction processes. Specifically,...
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