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Network structures formed by actin filaments are present in many kinds of fluorescence microscopy images. In order to quantify the conformations and dynamics of such actin filaments, we propose a fully automated method to extract actin networks from images and analyze network topology. The method handles well intersecting filaments and, to some extent, overlapping filaments. First we automatically...
A new probing system is proposed and developed to detect and analyze the interaction of bio materials at the molecular level. Unlike the conventional technique, when the probing method is used, the substrate is treated so that a single molecule is likely to attach to an isolated area. The substrate is micro-machined to have an Au nano-dot array with various sizes from 20 nm to 70 nm in diameter and...
This paper dealt with the following topics: molecular simulation techniques; microcontroller fabrication; genomic analysis; medical image processing; embedded system; medical information systems; sensor; wastewater removal; wearable real-time system; medical imaging; photovoltaic system; biomedical devices; ECG; BCI system; time concurrent hybrid functional petri nets; fuzzy system; gait analysis;...
We report investigations on the adsorption and in-vitro recrystallization kinetics of the bacterial surface layer protein of Bacillus sphaericus NCTC 9602 on gold surfaces by means of film bulk acoustic resonators. The acoustic resonators were operated in shear mode at about 800 MHz. From the measured changes of frequency and in dissipation, the mass and the viscoelasticity of biomolecular films formed...
Current cell detection techniques are antibody staining of specific protein markers, morphometric parameters and transgenic markers. These assays are often qualitative and do not quantitatively define the outcome of a cell progression during differentiation. Consequently, we propose to characterize the mechanical behavior of embryonic stem cell, which will predict its stage of differentiation during...
We report dual-color superresolution imaging using endogenously expressed fluorescent proteins. An imaging resolution of 20-30 nm facilitates study of the ultrastructural relationship between proteins present in adhesion complexes at the surfaces of whole, fixed cells.
A Fourier transform technique has been used to enhance the genome periodicities when analyzing the distributions of independent nucleotides and dinucleotides. These periodicities are varying from 2 to 500 bp. In this paper we focus on the 3 and 10.5 periodicities. The 3-base periodicity is characteristic for the protein-coding sequences only. The source of the approximately 10.5-base sequence period...
Summary form only given. Platelets adhesion occurs at focal adhesions (FA), where cell-membrane receptors bind specifically to substrate proteins and couple to each other and to the cytoskeleton via various cellular proteins. Some of the reactions that follow the ligand-receptor binding at the FA may affect mechanical determinants of the cell-substrate attachment. The resulting molecular structure...
A methodology is presented for simultaneous mechanical testing and atomic force microscopy imaging of single collagen fibrils under load. This method holds the promise for determining single-fibril modulus and strength in various experimental preparations. Examples of this utility include characterization of deformation and failure modes of naturally occurring and engineered structural proteins. Additional...
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