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Biofuels, such as butanol is a promising alternative to fossil fuels. In this case, novel cell factory may help in the production of butanol. However, this practice needs a good understanding in order to improve existing cell factories technologies for biofuels. System biology offers numerous methods using computational approaches to analyze and reveal the complex behaviors of biochemical reaction...
Biodiesel has been gaining increased attention as a nontoxic, biodegradable and renewable new green alternative diesel fuel. Production of biodiesel can be synthesized by chemical or by lipase methods, while lipase method has more advantages compared to chemical synthetic method. In this article, the microbial strains with activity of lipase were isolated from long time oil-contaminated soil and strain...
An experimental study was conducted that promoted the efficiency of anaerobic digestion by hydrolyzing pig manure in advance with amylase before loading it into the digester. A modified Gompertz equation was applied to determinate the kinetic parameters of biogas production. The results showed that the removal percentage of TS and VS, the biogas production potentials and rates have been improved through...
Used Tween-80 as emulsifler, industrial lipase as catalyst to hydrolyze waste oil from restaurants and make mixed fatty acid. We discussed the enzyme concentration, substrate concentration, temperature and time on hydrolysis. Through the experiment, we found that when we used emulsifier which contains 2% oil in the system, we could get the optimal parameter for hydrolysis of waste oil from restaurants...
In this research, results of an experimental study of prickly ash seed cust oil on the preparation of fatty acid methyl ester by lipase catalysis were presented. Factors such as n-hexane, the FAME rate, water concentration, volume of lipase, et al were studied. The experiment results showed that n-hexane is most suitable solvent in the non-aqueous lipase catalytic system and the FAME rate reach the...
A novel approach to the surface functionalization for membraneless enzymatic glucose/oxygen biofuel cell applications is described. The biofuel cell employs the gold plate electrodes modified by specific graphene-enzyme conjugations, which are immobilized by electrochemical deposition of the conducting polypyrrole polymer. The electrochemical activity of these electrodes is superior to the electrodes...
Formic acid hydrolysis is widely used in lignocellulose pretreatment. However, formic acid hydrolysis wheat straw cannot be directly used as a fermentation substrate owing to various fermentation inhibitors, especially the formic acid in reaction system and released during pretreatment. Study found treatment of wheat straw hydrolysate with D311 ion-exchange resin had a good result on reduction formic...
In this study, we produce biodiesel from jatropha oil through enzymatic synthesis in a re circulated packed bed reactor (PBR). A solvent free system by three stepwise addition of one equivalent molar methanol in each step was chosen for this study. The enzyme was Lipozyme IM, a 1, 3 regiospecific immobilized lipase from Mucor miehei. Initially, we scrutinized the appropriate amount of lipase by varied...
Pretreatment is one of the approaches to yield the higher saccharification process leading to optimization of ethanol yield. The effect of ultrasonic pretreatment on ethanol production from rice straw was studied. A 0.16 kW/L ultrasonic treatment was applied to treat rice straw during enzymatic hydrolysis and fermentation process. The enzymatic hydrolysis process was carried out at 50°C and pH 4.8...
Rice straw can be utilized to use as a raw material for production of biofuel due to its content with a high amount of sugar in a form of 35-40% w/w cellulose and 25-30% w/w hemicelluloses. The effect of ultrasonic treatment to enhance the saccharification process of the enzymatic hydrolysis from rice straw was investigated. Two frequencies at 20 and 24 kHz of ultrasonic treatment were used for 80...
Pretreatment of thickened waste activated sludge (TWAS) can facilitate the hydrolysis step of mesophilic anaerobic digestion (MAD) and improves the digestibility. This study examined the effects of microwave/alkali pretreatment on TWAS disintegration and its subsequent batch and semi-continuous MAD. The maximum solubilization ratio of volatile suspended solids, 77%, achieved at 210°C with 0.15 g-NaOH/g-TSS...
Deoiled Jatropha curcas seed meal (DJSM), a chief waste material of oil extraction industry, will be generated on a large scale due to the growing interest in using Jatropha curcas seed oil as a feedstock for the production of biodiesel. Up to now, there is very little work on viable approach for the use of DJSM to the best of our knowledge. This article reports our studies on the utilization of this...
The mechanically sorted organic fraction of municipal solid waste (MS-OFMSW) from Wuzhou was divided into above screen fraction (AS-MS-OFMSW) and below screen fraction (BS-MS-OFMSW). The experimental results showed that, the volatile solid (VS) of AS-MS-OFMSW was 28.6% with biodegradable VS of 82.5%. A VS removal of 19.7% and a methane yield of 93.1 L/kgVS were obtained in mesophilic digestion with...
Biodiesel production catalyzed by Novozyme 435 was optimized using Plaekett-Burman design and response surface methodology. Firstly, Plaekett-Burman was employed to screen the main efficient parameters on conversion rate from enzyme dosage, reaction temperature, tert-butanol amount, oil/alcohol molar ratio and molecular sleves added amount. Then, the steepest ascent experiments were carried out to...
The transesterification of Jatropha oil and methyl acetate to biodiesel catalyzed by Novozym 435 (N435) was explored. The organic solvent and the factors of influenced reaction were optimized.The optimum reaction conditions were 6% of N435 based on the Jatropha oil weight, the molar ratio of n-hexane to Jatropha oil was 19, 10% of organic trihydroxymethyl aminomethane base on the Jatropha oil weight,...
The activity behavior of Novozym 435 treated with organic solvents was investigated, and the influence of several crucial parameters, including Novozym 435 dosage, oil to methanol molar ratio, reaction time and type of monohydric alcohol, on biodiesel yield was also addressed using tert-butanol as co-solvent. The results showed that the functional group as well as log P of organic solvents was an...
8 strains producing lipase were isolated from soil with rich oil. Among them strain BD3 producing lipase was more powerful. Strain BD3 was identified as Pseudomonas sp according to its morphology, physiological, biochemical properties. We utilized PVA(polyvinyl alcohol) and Boric Acid as the vector of immobilized cells of Strain BD3, and have studied transesterification at the same time. We found...
Lipase catalyzed of biodiesel is expensive which restricted industrial enzymatic biodiesel production. In this paper, the industrial lipase made in China was used as catalyst to catalyze the transesterification of rapeseed oil with methanol to produce biodiesel. The way of methanol feeding, the amount of lipase, the reaction temperature, the reaction time, the molar ratio of methanol to oil, the organic...
The isolation and identification of a hyper-cellulytic fungus for cellulase gene selection was carried out as the initial stages in the production of a recombinant micro-organism. This would be capable of Consolidated BioProcessing (CBP) of cellulosic material to ethanol in single stage fermentations. With the selection of an organism with high cellulytic rate, the construction of a cellulytic ethanogen...
Biofuel cells are considered the most promising future energy source. This paper presents a bio-energy generation system based on the micro-fluidic platform which utilized enzymes as catalysts and glucose as a fuel to process the electrobiochemical energy conversion and storage in a cell. This is known as the enzymatic bio-fuel cell (EBFC). It is designed to consist of two modified gold electrodes...
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