Formic acid hydrolysis is widely used in lignocellulose pretreatment. However, formic acid hydrolysis wheat straw cannot be directly used as a fermentation substrate owing to various fermentation inhibitors, especially the formic acid in reaction system and released during pretreatment. Study found treatment of wheat straw hydrolysate with D311 ion-exchange resin had a good result on reduction formic acid. We investigated the influence of D311 resin content on the elimination of residual formic acid and the adsorbance of glucose. The results reveal that 22.5 g D311 resin can eliminate 94% of residual formic acid and with only 15% reduction of glucose concentration.