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G-quadruplexes are abundant locally stable structural elements in nucleic acids. The combinatorial theory of RNA structures and the dynamic programming algorithms for RNA secondary structure prediction are extended here to incorporate G-quadruplexes using a simple but plausible energy model. With preliminary energy parameters, we find that the overwhelming majority of putative quadruplex-forming sequences...
Computational tools are indispensable in the genetic engineering today. This research employs computational analysis on microRNAs which are targeting at the PTEN gene. This study aims to analyze the implication of microRNAs in the expression of PTEN which is leading to tumorigenesis. The analysis of microRNAs sheds light on the regulatory mechanism of PTEN in cancer development.
MicroRNAs are short regulatory RNAs which play a major role in regulating gene expression. In this study, we employed bioinformatics techniques to predict microRNA target sites for interleukin-17 family. We aimed at identifying the pattern of microRNA regulatory mechanism on interleukin-17 family. This study provides insight into the expression pattern of interleukin-17 family in controlling immunopathologic...
interferen-? of Rongchang porcine was amplifed by RT-PCR with the total RNA extracted from the peripheral blood lymphocytes. The gene was sequenced after cloned into the vector pMD18-T and analyzed with molecular biological software. The results showed that the opening reading frame (ORF) of interferen-? of Rongchang porcine consists of 501 nucleotides, encoding 166 amino acids, the front 23 amino...
Objective: To investigate the effect of ER-a36 siRNA on the growth of ER-breast cancer cell. Methods: The inhibitive effects of ER-a36 siRNA with various concentration on ER negative breast cancer cell at 24 h and 48 h were determined with MTT, the changes in DNA quantity was detected by Annexin V-FITC/PI staining, the expression levels of ER-a36 and pro-caspase-3 by Western blot. Results: ER-a36...
Regulation of gene expression has been shown to involve not only binding of transcription factor in target gene promoters but also characterization of histone around which DNA is wrapped around. Some histone modification, for example di-methylated histone H3 at lysine 4 (H3K4me2), has been shown to be associated with gene activation. However, no clear pattern has been shown to predict human promoters...
MicroRNA, as one typical type of molecule in RNA interference mechanism, plays a significant role in carcinogenesis. The identification of the silencing targets is one critical task. The bioinformatics tools predict enormous number of targets so as to be considered that most of the results are false positive. Target validation represents a great challenge to microRNA studies. We develop a systematic...
We established a novel expeditious drug screening approach, Feedback system control (FSC) with differential evolution (DE), to search for optimal drug cocktails that induced robust osteoblastogenesis of mesenchymal stem cells. The prototype drug cocktails composed of 7 independent factors including BMP-2 at 10 different concentrations were prepared to investigate the efficiency of FSC. As a trial,...
To investigate the effects of different doses of X-ray on the telomerase in human osteosarcoma (HOS) cells and to provide theoretical evidence for the clinical application of radiotherapy in osteosarcoma. Log phase HOS cells were radiated using 1, 2, 4 and 8 Gy doses of X-ray separately and cell survival was measured using MTT method, telomerase activity by ELISA, and telomerase reverse transcriptase...
We aimed to investigate the expression and location change of ER-a36 in BGC823 when dealt with TAM. Cell growth was examined by WST-1 and assayed by microplate reader. ER-a36 expressions in mRNA and protein were assayed by RT-PCR and Western-blot method and were quantitated. The location of ER-α36 in cell was examined by immunofluorenscence methods. The cell numbers significant decreased at all TAM...
RUNX3 is one of the suppressor gene in ovarian cancer, it is not clear whether the expression of RUNX3 is regulated by its promoter methylation or the demethylating agent 5-aza-2'-deoxycytidine. We carried out methylation — specific PCR (MSP) to investigate the methylation status of RUNX3 promoter region in human ovarian cancer cell line 3AO, KOV3 and normal ovarian epithelial cell NOEC. The methylation...
It is reported that circulating miRNAs are potential new biomarkers for diagnostic and other clinical applications. To study the varieties of plasma miRNAs expression, we investigated maternal plasma miRNAs expression profiles using SOLiD sequencing technology. The results showed that the distribution and abundance of specific miRNAs in plasma are varying during physiological pregnancy process. We...
Purposes: constructing prokaryotic vector of Timp3 (tissue inhibitor of metalloproteinase-3). Methords: Total RNA of human placenta tissues were prepared, and the cDNA encoding Timp3 was got via RT-PCR. The gene was constructed into pMD18-T vector using the restriction enzyme of BamHI and XbaI. Results: By agrose gel electrophoresis and DNA sequencing, Timp3 gene was introduced into pMD18-T successly.
In order to explore the effect of paclitaxel (PTX) combined with Survivin siRNA on proliferation and apoptosis in U-87MG cells in vitro, the U-87MG cells were transfected with Survivin siRNA and treated with PTX at the same time. The cell morphological changes were determined by methylene blue solution. Survivin, cyclin D1, c-Myc and CDK4 expression in U-87MG cells was analyzed by RT-PCR and western...
Objective: To discuss the effects of Chrysan them um m orif olium on mRNA expressions of p53 and Bax in the Human Umbilical Vein Cells(HUVEC) under Induction and Cultivation of high glucose. Methods: Make four groups of the HUVEC as normal cells group, normal glucose - only deal cells group high glucose -only deal cells group and Chrysanthemum morifolium + high glucose acting cells group. After 18∼24h,...
Nearly 40 hereditary disorders in humans result from an increase in the number of copies of simple DNA repeats in genomic DNA. DNA repeats expansion mutations are dynamic and ongoing within tissues and across generations, leading to disease. These DNA repeats seem to be predisposed to expansion because they have unusual structural features, such as hairpins, slipped-strand DNA, DNA unwinding elements,...
Conditions of the extraction of polysaccharides from protocorms of Dendrobium candidumwere optimized by orthogonal experiment. The results showed that the optimum technology of isolation is to extract about 3 times and 0.5 hours each time in 30 times water at 100°C, the ways to break cell is high-speed homogenate. After 24h, the mRNA expressions of NF-κB was detected by reverse transcription-polymerase...
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