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Lactate dehydrogenases, LDH, catalyzed reaction has been used in this chapter as a conductor wire to present the evolution and difficulties on computing methods to model chemical reactions in enzymes, since the early calculations based at semiempirical level carried out in gas phase to the recent sophisticated simulations based on hybrid Quantum Mechanical/Molecular Mechanics Dynamics (QM/MM MD) schemes...
This study reports the preliminary development to biosensor using electrochemistry for lactate analysis. In this work, PEDOT/PSS modified screen printed carbon was developed as working electrode with lactate dehydrogenase (LDH), the oxidized form of nicotinamide adenine dinucleotide (NAD+) and glutaraldehyde. The result shows that the current from working electrode with PEDOT/PSS were higher compared...
Glucocorticoids are considered to be indispensable agents in the treatment of hematologic malignancies. A critical established glucocorticoid action is the apoptotic effect they exert on leukemic cells. However, little is known about the molecular response of malignant cells on glucocorticoid exposure. Even less is known about the cell proliferation dynamics governing leukemic cells under glucocorticoid...
Analysis of blood biochemical parameters is an important aspect of toxicological studies in fish. Anesthetic is typically used before blood sampling to minimize injury to the fish; however, anesthetic may also act as a stressor and alter biochemical measurements. To better understand blood responses to anesthetic stress, we evaluated biochemical alterations in the blood serum of endangered Chinese...
To determine whether modulating reactive oxygen species (ROS) release by mitochondrial is involved in the cardioprotection exerted by acetylcholine (ACh). In isolated ventricular myocytes from male Sprague-Dawley rats, 0.1 ??M ACh was administered for 6 min before 60 min of simulated ischemia and 30 min of reoxygenation or reperfusion (I/R). A mitochondrial ATP-sensitive potassium channel (mitoKATP...
In vivo, cardiomyocytes are stimulated by electricity continuously. So electrical stimulation is an indispensable factor for cardiomyocytes cultured in vitro. In this study, the experimental group neonatal cardiomyocytes received electrical stimulation at different intensities and different frequencies by a electrical stimulation device in vitro. After stimulation, The morphology and beating of cardiomyocytes...
In this study, the 5' terminus of ldhL gene in Lactobacillus casei ATCC 334 was fused to the 3' terminus of pdc gene promoter (Ppdc) part in Zymomonas mobilis ATCC 10988 by recombinant PCR. The fusion gene Ppdc-ldhL was integrated in broad-host-range plasmid pBBR1MCS3, transformed by electroporation and expressed in Z. mobilis ATCC 10988. The primary expression of heterologous lactate dehydrogenase...
Toluene diisocyanate was a kind of indoor air pollutant with low toxic, but the most toxic in all diisocyanate esters, which was recognized as a kind of industry allergen. To study the effect on markers of oxidative damage and energy metabolism in blood induced by toluene diisocyanate(TDI). The mice contacted with TDI by respiratory tract in 50L static inhalation tank. The Exposure sustained two weeks,...
With development of nanotechnology application, industry and government regulators notice that nanomaterials, especially nanoparticles, have some potential intimidation for human health and environmental safety. In this work, the cytotoxicity of 20 nm and 200 nm polystyrene nanospheres was investigated in cultured L929 fibroblast cells. There was an obvious difference of cytotoxicity between 20 and...
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