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Today, chromatography is the foundation of detachment science and is being utilized as a part of all examination research centers and pharmaceutical enterprises of the world. In these chromatography Ultra Performance Liquid Chromatography (UPLC) is a moderately new procedure giving new conceivable outcomes in fluid chromatography, particularly concerning abatement of time and dissolvable utilization...
In this paper, a quantification method of the carbohydrate is proposed for samples of spot tests using the thin-layer chromatography (TLC) based on the scanned image analysis compensating lack of overlap between each spot. The color density of image data is modeled by 2-dimensional Gaussian function. Parameters in the Gaussian function are estimated by calculating marginal distribution and using least...
As new electrode material, boron doped diamond (BDD) electrode has the characteristics of high oxygen evolution potential, adsorption inertia, good physical and chemical stability, thus it might be very promising in advanced treatment of organic wastewater. The strong oxidation ability of BDD electrode is mainly attributed to the hydroxyl radicals, which can degrade organics efficiently, and occupy...
The most abundant compound in the Nephelium lappaceum leaves extract is gallic acid, a phytochemical with antioxidant and anticancer properties. In order to test its anticancer activity, human liver (HepG2) and lung (A549) cancer cell lines were cultured and tested with different concentrations of the crude extract. To study its cytotoxicity on normal epidermal cells, the extract was tested on K38...
It is well-known that carbohydrate sugar chains, or glycans, play various well in cellular processes, including cancer, but the elucidation of glycans is difficult because of their complex structure. Both computational methods and mathematical models are necessary to integrate and analyze the information of glycomics data so as to efficiently detect glycan structures. In this paper, we propose a new...
Vine shoots are viticulture byproducts recently valorized as important sources of polyphenols. These are powerful natural antioxidants used in several industrial applications. The aim of this work is to intensify polyphenol extraction from vine shoots (Syrah variety) using energy saving techniques. In this study, we chose to work with green solvent and in absence of organic solutions. The effect of...
Benzimidazole derivatives are wide interest because of their biological activity and clinical uses. Short and simple method was developed to control the "carry over impurity" in 5-methhoxy-2-mercaptobenzimidazole. This benzimidazole derivative was used in various drug substance syntheses. The proposed method was short (20 min run time) and validated as per the standard validation guidelines...
In this study it was aimed at evaluating antioxidant potential of Penicillium sp. broth filtrate and extract. In our study we examined broth filtrate DPPH and free radical scavenging activity, and also total phenol content (Folin ciocalteu reagent). We used 38 fungi samples in our study. We selected free radical scavenging activity over 70% in one fungi among them. In parallel with these results total...
Normally pineapple index 2 are harvesting for canning process. Pineapple waste is a by-product from the pineapple canning processing that will lead to serious environmental pollution. Some of pineapple waste is used for animal feed but it is still under utilized. Pineapple waste contains valuable nutrient component of simple sugar such as sucrose, glucose and fructose. Analysis of physico-chemical...
Xanthone is one of the major bioactive secondary metabolites in Garcinia mangostana. A simple reversed-phase high performance liquid (RP-HPLC) which equipped with photodiode array detector has been developed to analyze xanthone in rat plasma. The rat plasma was treated with deproteining agent (DA) consists of acetonitrile: propanol (1:1), in the ratio of 2:1 (DA: samples). The mobile phase comprises...
Levodopa (l-dopa), the metabolic precursor of dopamine, has primarily been used for the treatment of Parkinson's disease (PD). A reversed phase high performance liquid chromatography (RP-HPLC) method with UV-detection at room temperature has been developed for analysis of levodopa. The method entailed direct injection of rat plasma sample after deproteinization using acetonitrile — propanol (1:1)...
To establish a sensitive HPLC method for determination of gossypol-acetate (GA) enantiomers in rat plasma. The study involved administration of oral and intravenous to rats. Plasma samples were collected before and during the predetermined time intervals following administration of GA enantiomers, which were analyzed using HPLC method. The method showed a good linear relationship in the calibration...
This research paper reports a novel non-invasive electrochemical technique that is used to elucidate the function of the pancreas. The pathology of the pancreas is not easy to elucidate due to its anatomical location. Various invasive tests are done such as Endoscopic Ultrasound and Endoscopic retrograde cholangiopancreatography (ERCP) to determine its function but they have side effect and are not...
A simple, rapid and reproducible HPLC method was used for the simultaneous determination of chlorogenic acid, baicalin and forsythin in Xiao'erjiebiao Granules, at the same time, response surface methodology (RSM) was devoted to forecast the best extraction technology. The HPLC System included an Agilent ZORBAX SB-C18 column ( 250 mm × 4.6 mm, 5µm ), acetonitrile (A) -- 0.4% ethylic acid (B) as mobile...
A high performance liquid chromatography (HPLC) method was established for determining contents of seven effective constituents (orientin, isoorientin, vitexin, isovitexin,chlorogenic acid, caffeic acid and ferulic acid) in Bamboo leaf from different provenances of Phyllostachys. Seven effective constituents were simultaneously determined by HPLC with Sunfire C18 ODS column ( 4.6 mm×250 mm, 5µm )...
In this paper, soy isoflavones as raw material, the use of acid hydrolysis of cellulose daidzein, soybean isoflavones by HPLC method and daidzein content of the orthogonal design, the system of the substrate concentration, enzyme concentration, conversion time on the soybean isoflavone daidzein into effect, the best single-factor test verification transformation temperature, pH, to determine the optimal...
A HPLC method was developed to determine the concentration of schisandrin in plasma and tissues of mouse. The separation was obtained on a C18 column with a mobile phase of methanol: water (58:42, v/v), UV detector was monitored at 250 nm. The established method was successfully used to the research of pharmacokinetics and tissue distribution of schisandrin after intragastric administration of a 15...
A HPLC method for the determination of the content of Isoniazid and Isonicotinic acid in Isoniazid Tablets was established. The determination was performed on SHIM-PACK VP-ODS C18 column. The mobile phase consisted of (40:60) methanol-water (0.2%Docusate Sodium) at a flow rate of 0.8ml·min−1. The UV detection wavelength was set at 261nm and the sample size was 5μL. The linear range of isoniazid and...
The enantiomeric separations of three neonicotinoid insecticides (compounds 1, 2, and 3) were performed on a Chirobiotic V chiral column by high-performance liquid chromatography (HPLC) under a new-polar mobile phase mode, using methanol and ethanol with triethylamine as the basic additive as the mobile phase. Compound 1 obtained no separation under all the mobile phase, while compound 2 and 3 were...
Phytoplankton pigment composition has been studied during two years at station located in the subtropical North Atlantic Ocean, in a station for time series in the ocean. The concentration and proportion of several diagnostic pigments is used for the determination of phytoplankton communities present in the area, and consequently their seasonal and temporal variability. The knowledge of phytoplankton...
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