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The interaction of bovine serum albumin (BSA) with tetraphenyl-porphyrin derivatives such as BrTPP and CH3OTPP was investigated by fluorescence spectroscopy. The influence of electron effect of substituting group on fluorescence quenching mechanisms was discussed. The number of binding sites n, and the binding constant KA and thermodynamic function were obtained. The effect of porphyrin on the conformation...
The fluorescence and polarization spectra of isopropanol-water solution excited by ultraviolet light and linearly polarized light are studied experimentally. The fluorescence spectral characteristics and emission mechanism are discussed. The molecular clusters information in different concentration and polarization property are analyzed. It is found that the isopropanol-water solution obtains the...
Pretreatment and determination for mercury species based on coupled chromatography techniques especially the combination of liquid chromatography with atomic fluorescence spectrometry were reviewed in this paper with 17 references, and the new trends for trace and ultra-trace of mercury species analysis in the future was also carried out.
Chloroethylnitrosoureas (CENUs) were an important family of anticancer agents in the clinical treatment of human malignancies. Several lines of evidences indicated that their cytotoxicity was related to the interstrand crosslink of DNA. In this study, a method for the determination of DNA crosslink was established using real-time fluorescence quantitative PCR. The DNA crosslink ratio induced by two...
A new catalytic dynamic spectrofluorimetry method is proposed for determination of trace tin which is based on the reaction between potassium periodate and the new type fluorescent reagent 3-m- chlorophenyl-5-(2'- arsenoxylphenylazo) rhodanine (3CIRAAP). The fluorescence intensity is investigated to be sharply quenched by the oxidation of 3-m-chlorophenyl-5- (2'-arsenoxylphenylazo) rhodanine by potassium...
Conjugated polymer based on thiophene having high reasonably quantum yield has been synthesized. A bulky butoxycarbonylmethyl urethane moiety as a side group is attached to the polymer backbone. The polymer is characterized by 1H NMR, FT-IR, UV-Visible absorption and fluorescence spectroscopy. Thin film fluorescence sensors based on this material were fabricated through a spin-coating technique. The...
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