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Imaging bio-molecules especially in tissues, cells and even in in-vitro live conditions require expertise not only in staining and tagging of the particular molecules of interest but also their imaging so as to make any meaningful analysis and quantification of the obtained images. This necessitates a microscopic facility which can grab fluorescent images with minimum bleaching of the dyes and gives...
Clinical translation of scientific discoveries is often the long-term goal of academic medical research. However, this goal is not always realized due to the complicated path between bench research and clinical use. In this review, we outline the fundamental steps required for first-in-human testing of a new imaging device, and use the FLAREtrade (Fluorescence-Assisted Resection and Exploration) near-infrared...
Fluorescence microscopy allows the acquisition of the spectroscopic properties of fluorescent reporter molecules at levels of resolution too small to be seen with the naked eye. The Indirect Immune Fluorescence Test is the method used to identify antinuclear antibodies. The main principle of this method is to identify the auto-antibodies in a patient's blood serum by staining affected cell structures...
We report the development of a diagnostic system combining time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy and its application in diagnosis of tumors and atherosclerotic disease. This system allows for concurrent evaluation of distinct compositional, functional, and micro-anatomical features of normal and diseased tissues.
Membrane dynamics plays an essential role for cellular processes. Here we demonstrate the application of several biophotonic techniques, such as advanced fluorescence microscopy and laser spectroscopy, to study some fundamental membrane biology problems.
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