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We present a portable microendoscope based on a microelectromechanical systems (MEMS) scanner, compound gradient refractive index micro-lenses and a photonics bandgap fiber (PBF). It overcomes the size (2.0 × 1.9 × 1.1 cm3) and weight (less than 3 grams) limitations of conventional two-photon fluorescence microscopy toward freely moving subjects. The microendoscope utilizes a PBF for laser excitation...
In this paper we present an approach based on mathematical and computing aspects of a method for the correlation of images representing investigation results over the same area of an object. The aim of this algorithm is to associate the results with a three-dimensional digital model of the investigated surface in an accurate manner. Thus a digital replica can be obtained with unified and complementary...
Far-field fluorescence microscopy is the most frequently applied microscopy technique in life sciences. Its strength is the unique combination of highly attractive features such as molecular specificity, simple sample preparation, possibility of 3D imaging and operation under ambient, live cell compatible, conditions. The main shortcoming in comparison to methods, such as electron microscopy, is its...
The authors present a short review on different microscopy techniques based on laser scanning microscopy. The corresponding wavelengths of the techniques cover the range from between far field (hundred nanometres) and near field (few nanometres). Our investigations started with a hundred nanometers resolution in the case of laser beam induced current technique and go to nanometre resolution in the...
Optical Coherence Tomography (OCT) is a well known technique for creating non-invasive, high resolution images of biological microstructure. OCT has matured into animportant imaging modality since its invention in 1991. The popularity of the method lies in the numerous advantages that it offers high detection sensitivity allowing fast imaging speed, andhigh spatial resolution. In this paper we propose...
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