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Acoustic droplet vaporization (ADV) has shown a great potential in extravascular tumor-targeting theranostics. During ADV, its mechanical effects on the nearby cells could cause cell dysfunction with irreversible cell membrane damage or facilitate drug delivery by sonoporation. Understanding the real-time characteristics and bio-effects of ADV on cells is significant to develop the approach in vivo...
We describe the fabrication process for an anode of a miniaturized transmission electron microscope. The anode consists of a very thin membrane (membrane-anode) that encloses the electron optics microsystem and it is used to separate the high vacuum part from the sample chamber in the microscope. The membrane-anode is made of Si3N4 layer deposited on an oxidized silicon substrate and was fabricated...
We measured the membrane roughness of neuroblastoma cells under the treatments of gold nanoparticles and paclitaxel. The stabilization of microtubules by paclitaxel reduced the membrane roughness. The positive charges on nanoparticles led to similar results.
Cell culture on a silicon nitride membrane is required for atmospheric scanning electron microscopy, electron beam excitation-assisted optical microscopy, and various biological sensors. Cell adhesion to a silicon nitride membrane is weak, and the proliferation potential is also limited. Therefore, we considered that the adhesion force and proliferation potential of cultured cells might be increased...
Morphological and topological changes of biological membranes play essential roles in cellular activities, such as endocytosis and exocytosis. Here, real-time imaging of giant liposomes using optical dark-field microscopy reveals that the lipid bilayer membrane possesses the ability to undergo topological transformation through interactions with proteins, peptides, or surfactants.
We present an automatic approach for 3D segmentation of mouse embryonic stem cell nuclei based on level set active contours. Due to the specific properties of these cells, standard methods for cell nucleus segmentation and splitting of cell clusters cannot be applied. Our segmentation approach combines information from two different channels which represent the nuclear region and the nuclear membrane...
In this Letter, the nanoporous anodic alumina membranes (nanoPAAM) were first fabricated by the two-step anodic oxidisation method, and then based on these nanoPAAM master molds, polymer polymethylmethacrylate nanopillars with controllable heights and diameters were achieved by the direct casting method. Subsequently, combined with the ultraviolet lithography or electron beam lithography, a kind of...
Carbon nanotubes (CNTs) have been widely investigated as one of the most promising nanomaterials in biomedical applications. Functionalization of CNTs with poly(ethylene glycol) diamine (PEG) is a recognized methodology with good solubility and biocompatibility. In this study, PEG conjugated (PEGylated) multi-walled CNTs (MWCNTs) were prepared and labeled with fluorophore FITC. Using fluorophore-conjugated...
In order to combine the advantages of silica as an inorganic material (chemical resistance, optical properties) to that of polypyrrole as a conductive polymer one has attempted to obtain novel silica-polypyrrole composite particles by a newly improved technique consisting to in situ polymerization of adsorbed pyrrole into silica. The new synthesized material was characterized by FT-IR spectroscopy,...
We propose a novel Markovian segmentation model which takes into account edge information. By construction, the model uses only pairwise interactions and its energy is sub modular. Thus the exact energy minima is obtained via a max-flow/min-cut algorithm. The method has been quantitatively evaluated on synthetic images as well as on fluorescence microscopic images of live cells.
Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. The...
To discuss the mechanism of the apoptosis induced by n-butanol extract from Capparis spionosa L. (CSBE) in human heptocarcinoma cell Line HepG-2, Laser Scanning Confocal Technology and Flow Cytometry were used to investigat the changes of mitochondrial membrane potential and Ca2+ levels in HepG-2 cells. The changes of mitochondria membrane potential induced by CSBE on the HepG-2 cells were studied...
The purpose of this article was to observe interaction between drug and cancer cell with fluorescence probes and molecular imaging technology. In order to study the effect of cell growth and apoptosis morphology of Human gastric adenocarcinoma SGC-7901 induced by n-butanol extract from Capparis spionosa L. (CSBE), laser scanning microscopy was employed to observe apoptosis imaging. In addition, the...
To study the effect of Saponins of asparagus on HepG2 apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (Δψm) levels. Saponins of asparagus with different concentration was treated with HepG2 at different time, MTT assay was used to detect inhibitory rate, fluorescence staining was used to observe apoptosis morphology, flow cytometry was used to detect apoptosis rate and...
The purpose of this article was to observe interaction between drug and cancer cell with fluorescence probes and molecular imaging technology. In order to study the effect of cell growth and apoptosis morphology of Human gastric adenocarcinoma SGC-7901 induced by n-butanol extract from Capparis spionosa L. (CSBE), laser scanning microscopy was employed to observe apoptosis imaging. In addition, the...
The model of different level sub-chronic aluminum (Al)intoxication in chickens was established by continuous peritoneal injection of various gradient aluminum trichloride (AlCl3). The growth index of cerebrum was measured and the morphological structure of it was observed by optical microscope and transmission electron microscope. The results showed that:(1)The growth index of cerebrum was lower in...
Background: CD44v6 is a splice variant of CD44 (CD44v) could probably promote cancer cells to adhere to vascular endothelium and base membranes and enhancing the invasion and metastasis of colonic carcinomas. Heat shock protein 72 (HSP72) as a molecular chaperone has been confirmed to overexpress in epithelial carcinoma cells. There may be a possible correlation between the expression of HSP72 and...
In order to investigate the effects of electric pulses on cancer cells, HepG2 and L02 were exposed to electric pulses (1000V/cm, 100μs, 1Hz) for different time (8s, 15s, 30s, 60s). Annexin-V-FITC Kit and Flow cytometry were used to study the apoptosis of treated cells. The results showed that the electric pulses of 1000V/cm, 100μs, 1Hz for 8s couldn't induce tumor cells apoptosis. Apoptosis was observed...
Liposomes have a wide variety of applications in Biomedical Engineering, such as in vaccines, genetic engineering, anti-cancer drugs and drug controlled release systems. Traditional preparation methods produce liposomes with a wide size distribution and elaborate processes are needed to produce narrow size distributions. The research presented here, attempts to improve the traditional preparation...
HER-2/neu, a protein often giving higher aggressiveness in breast cancers, has been shown that if the gene is expanded for some reason, the Her-2 protein produced by the cells will be over-expressed to enhance the cancer cells reproduced ability, the prognosis will be also relatively less, too. The HER-2 immunohistochemical stained provides a simple and reliable method for pathologist in clinical...
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