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Research presents regularities investigation in the atomic structure of amorphous rapidly quenched Co58Ni10Fe5Si11B16 at.% alloys. The alloys were quenched on a copper wheel with a linear surface velocity from 22 to 38 m/s. We found nonlinear dependence of local atomic ordering from linear velocity of cooling wheel. Average lateral density of ordered atomic clusters of 5 nm size changes from 4% to...
Adhesives are used nowadays in an uncountable number of applications in everyday life, especially applications where a failure in service may lead to economic loss, injury, or death. In these cases, the ability to determine the causes of failure is essential. This chapter discusses the latest forensic engineering techniques used in the investigation of failed adhesive-bonded joints. An overview of...
Cryo electron microscopy records essentially projection images of each of many instances of a nano bio object. This data allows reconstruction of a stochastic model of the object, i.e., the mean and covariance functions of the electron scattering intensity of the object. Understanding the covariance function, which characterizes the heterogeneity of the instances of the object, is challenging because...
We present a new automated neuron segmentation algorithm for isotropic 3D electron microscopy data. We cast the problem into the asymmetric multiway cut framework. The latter combines boundary-based segmentation (clustering) with region-based segmentation (semantic labeling) in a single problem and objective function. This joint formulation allows us to augment local boundary evidence with higherlevel...
In this work, we present an image database for automatic bacilli detection in sputum smear microscopy. The database comprises two parts. The first one, called the autofocus database, contains 1200 images with resolution of 2816 × 2112 pixels. This database was obtained from 12 slides, with 10 fields per slide. Each stack is composed of 10 images, with the fifth image in focus. The second one, called...
In this paper, we describe the plasmonic and plasmon-photon coupling properties of nanostructured metallic films obtained by a self-assembly protocol. A gold layer is deposited on top of a self-assembled deposition of silica beads (artificial opal), which thus acts as a template. Atomic-force and scanning-electron microscopies demonstrates a periodic pattern on the metal surface with groove depth...
Mitochondria play an important role in cellular physiology and synaptic function. Recent electron microscopy (EM) advances make it possible to observe mitochondrial structure on nanoscale, but the attendant massive EM data unfortunately requires months of tedious manual labor. In this paper, we present an automatic approach for the 3D reconstruction of mitochondria from anisotropic EM stack. We first...
The monitoring of the state of the insulation and it's interpretation is an important and challenging task. In order to monitor the state of the insulation after application of various voltages under DC and AC conditions, the samples of the paper insulating material Leatherite paper was observed under Scanning Electron Microscope (SEM). The SEM (JEOL JSM-6480LV) was operated in low vacuum mode in...
Scientific education is strongly based on laboratory activities, but today laboratories are radically different with respect to their 20th century precursors. Computer technologies, digital networks and on line learning platforms are exploited increasingly in order to publish teaching materials, course calendars, class news and to support on line discussions, assignments and reviews even for face...
This paper presents a computational approach for detection of filamentous networks in 3D tomographic electron microscopy. Due to the general heterogeneity of chemical staining, imaged signatures may appear punctate and discontinuous. Very often, there is a necessity to characterize organization and phenotypic signatures of stained structures. This is the example of polysaccharides in the plant cell...
Recent publications questioned the validity of endothelial cell (EC) culture studies of glycocalyx (GCX) function, due to findings that GCX in vitro may be substantially thinner than in vivo. The assessment of thickness differences is complicated by GCX collapse during dehydration for traditional electron microscopy. We measured in vitro GCX thickness using rapid freezing/freeze substitution transmission...
This contribution presents a method for automatic detection of excitatory, asymmetric synapses and segmentation of synaptic junctional complexes in stacks of serial electron microscopy images with nearly isotropic resolution. The method uses a Random Forest classifier in the space of generic image features, computed directly in the 3D neighborhoods of each pixel, and an additional step of interactive...
Ferromagnetic hysteresis and coupled dynamics in the magnetic lens system of electron microscopes degrade the machine's performance in terms of steady-state error and transition time. To get a clear understanding of the exact problem and the way it is expressed in the application a commercial scanning electron microscope is extended with a data-acquisition and rapid proto-typing system. By means of...
In addition to statistically relevant standard reliability tests and lifetime analysis, the study of solid-state physical degradation mechanisms for a limited number of representative samples is needed to understand weaknesses in the interconnect technology and to exclude reliability-related failure in Cu interconnects. We present dynamic studies of damage mechanisms in on-chip Cu interconnects caused...
In single-particle reconstruction methods, projections of macromolecules at random orientations are collected. Often, several classes of conformations or binding states coexist in a biological sample, which requires classification, so that each conformation can be reconstructed separately. In this work, we examine bootstrap techniques for classifying the projection data. When these techniques are...
In this paper, we apply iterative transform algorithms, namely the error reduction algorithm and the hybrid input-output algorithm, to retrieve missing data in 3D reconstruction from 2D crystal images. 3D protein structures are determined using cryo-electron microscopy (cryo-EM). Extremely strong noise in cryo-EM brings in unreliable artifacts and a limited number of projections leave missing components...
MEMS electron-transparent membranes made of low-stress silicon nitride are widely employed in electron microscopy. However, this material has limited resistance to electron beams. We therefore developed a layer of LPCVD SiC. Our layer is amorphous, uniform, continuous, low-stress, and has extremely low etch-rates in common wet etchants. As free-standing electron transparent window, it demonstrates...
Far-field fluorescence microscopy is the most frequently applied microscopy technique in life sciences. Its strength is the unique combination of highly attractive features such as molecular specificity, simple sample preparation, possibility of 3D imaging and operation under ambient, live cell compatible, conditions. The main shortcoming in comparison to methods, such as electron microscopy, is its...
Kinetics of two dimensional islands on Si(111) was investigated by in situ ultrahighvacuum reflection electron microscopy during sublimation. The temperature dependences of critical radius of the terrace for nucleation of new island were measured. On the base of the obtained data the adatom diffusion length on the Si(111) surface was estimated at temperature range 1000 - 1300degC.
To better understand the central nervous system, neurobiologists need to reconstruct the underlying neural circuitry from electron microscopy images. One of the necessary tasks is to segment the individual neurons. For this purpose, we propose a supervised learning approach to detect the cell membranes. The classifier was trained using AdaBoost, on local and context features. The features were selected...
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