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The progress in the development of a piezoelectric immunosensor for the detection of a secretion protein (38 kDa) from Mycobacterium tuberculosis are described. A high affinity and specificity monoclonal antibody was obtained from a recombinant form of the target protein. This immunoreagent was employed for the development of a competitive ELISA, as a previous diagnostic test and reference assay for...
An 8-pixel quartz crystal resonator array with a fundamental resonance frequency of 73 MHz has been designed and fabricated. We established a protocol for selectively functionalizing the surface of resonator array via electrochemical desorption. The creation of hydrophobic and hydrophilic surface with methyl terminated and carboxylate terminated alkanethiols is characterized by Fourier transform spectroscopy...
The mass sensitivity (>; 1 micrograms) of current commercial analytical instruments, such as thermal gravimetric analyzers, severely limits their utility for measurement of valuable but poorly soluble materials such as synthetic proteins or DNA fragments. A quartz crystal microbalance (QCM), based on a transverse shear mode piezoelectric crystal operating at high frequencies, is gaining popularity...
Rutherford Backscattering Spectrometry (RBS) using 6 MeV alpha particles and X-Ray Fluorescence (XRF) using a Pd-anode X-Ray generator were performed to characterize Au and Pt contacts deposited by electroless technique and thermal evaporation on differently treated surfaces of CdZnTe and CdTe crystals. The aim of this study is to understand and improve the structure of the material-electrode interface...
Piezoelectric quartz crystal impedance (PQCI) analysis was applied to study the binding of L-tryptophan to human serum albumin (HSA), which was immobilized on the matrix of bare gold elelctrode/chitosan/glutaraldehyde/cysteamine/nanogold particles to maintain biocompatibility, increase capacity and allow renewing the electrode. The real-time course of the resonant frequency and equivalent circuit...
Experiments and modeling have been used to investigate the transport in vertical spin coated TIPS-pentacene layers used in rectifying diodes. It has been shown that the vertical transport properties are not as good as the longitudinal one, due to the particular nature of the polycrystalline film obtained by this process.
Self-assembled monolayer (SAM) techniques offer a new strategy for the immobilization of antibodies, antigens and DNA on the surface of quartz crystal electrodes. The quartz crystal microbalance (QCM) system provides a unique opportunity to monitor, in-situ and in real-time, the rate of adsorption of ethanthiol onto gold. The change in frequency resulting from the adsorption of ethanthiol molecules...
In this paper the authors describe the development and the characterization of a Quartz Crystal Microbalance (QCM) sensor for NO2 detection with the sensitive layer made of a thin films of poly (3,4-ethylenedioxythiophene) (PEDOT). The PEDOT layer was obtained by electropolymerization on one of the gold electrodes of a 10 MHz AT-cut commercial quartz crystal. The sensor performance in terms of sensitivity...
Recently, there has been interest in the fabrication of multiple Quartz Crystal Microbalances (QCMs) on a single substrate, to create a sensor array. The QCM's are ldquowell structuresrdquo etched on an AT-cut quartz substrate, to isolate the devices from one another. However, such devices are ultimately subject to the limitations of the QCM configuration, requiring electrodes and wires on the sensing...
A comparison of long-term stabilities of TlBr detectors with Au and Tl electrodes was performed in this study in order to reveal polarization phenomena in TlBr detectors. Au/TlBr/Au detectors exhibited polarization phenomena at room temperature observed as degradation of spectral performance and reduction of leakage current with elapsed time. Applying reverse bias voltage to the detectors recovered...
Nanowire arrays of CIS were formed inside porous alumina templates by pulsed potential electrodeposition. Close to stoichiometric CIS phase in a chalcopyrite crystal structure with a = 5.782 Å and c = 11.619 Å. was obtained. Atomic weight fractions of Cu, In and Se were 20%:38%:42%. Arrays of cadmium sulfide nanowire were also fabricated inside the porous AAO templates. The XRD pattern of the CdS...
Gold electrode quartz crystal microbalances (QCMs) were used as transducing platform to detect and sense elemental mercury (Hg) in gas phase. The enhanced sensitivity, resulting form the electro-deposition of gold nano-structures on the surface is shown to increase the response magnitude (RM) of the sensors in the presence of ammonia and humidity interference. The concentration of NH3 and H2O was...
We have constructed a piezoelectric sensor matrix with the capability of measuring the flexibility of immunoglobulin. The matrix consisted of 6 independent quartz crystal microbalance (QCM) coated with gold nanoparticles (GNP) of 3.5, 5, 12, 17, and 37 nm, and BSA. The detection was performed simultaneously. Antiserum against GNP bound to QCMs coated with 3.5 nm GNP and maximized for 5 nm GNP-coated...
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