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In this poster, unique nanobioconjugation techniques to produce targeted nanoprobes with high fluorescence activation, establishing a highly enhanced fluorescence sensor system are presented. By diversifying conjugation styles to the AuNP surface, a ratio of targeting agents and dyes can be achieved for 100 time fold activation. Conjugation of fluorescence dyes for quenching is dependent on access...
This study presents an integrated microfluidic system capable of rapid diagnosis and subtyping of influenza virus. Several critical modules including a ribonucleic acid (RNA) extraction module, a reverse-transcription polymerase chain reaction (RT-PCR) module and an optical detection module were integrated into the microsystem. Magnetic beads conjugated with specific nucleotide probes were first incubated...
The unique photophysical properties of semiconductor quantum dots (QDs) have made them ideal for use as spectral labels and luminescent probes. In this review, applications are presented in which QDs function as active participants in nanoscale biosensor assemblies, where replacing traditional molecular fluorophores results in improved assay performance. Specific focus is on disease detection with...
The use of biological ??nanopores?? as sensors for single molecules like DNA was recently demonstrated: the passage of the molecules through the pore separating two electrolyte-filled reservoirs is induced by a voltage application and detected as a current drop. Present attention is devoted to solid state nanopores, with obvious advantages, such as high stability, control of diameter, adjustable surface...
There is a considerable effort directed towards understanding and negating the proliferation of infectious diseases by improving the sensitivity of biosensors. We describe the investigation of nucleic acid interactions based on immobilization of thiolated probe DNA followed by the coupling of biotinylated DNA and streptavidin coated Dyna beads on the gold film deposited on the substrate for the further...
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