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In this paper software for processing an output signal from microfluidic devices with optical detection is presented. Such microdevices (lab-chips) are utilized e.g. in rapid analysis of DNA, proteins and cells and mainly utilize fluorometric detection technique. Here we present dedicated software for smart signal processing and automatic recognition of fluorescence in silicon-glass and entirely glass...
An optical particle detection and analysis system is presented. This platform combines the capillary microfluidics, integrated optics and novel image acquisition and analysis algorithms to form the basis of a portable or handheld cytometer instrument. Experimental results provided shows the testing results are closely matched with conventional flow cytometer data.
Controlled release of drugs with nanostructured capsules in microdroplets (μdroplets) using a microfluidic device is reported for the first time. Specifically, experiments demonstrated successfully that the drug release can be controlled by dynamically changing the pH of the control solution (water + NaOH) through a microfluidic chip. By observing the fluorescent images of microdroplets, it shows...
Sorting of specific target cells is an important process in biotechnological research and clinical medicine. This paper proposes a methodology that integrates optical tweezers and microfluidic chip technologies to realize the automatic cell sorting in a continuous flow environment. In the proposed system, cells are driven through the region of interest, and the digital image processing technique is...
In the paper a discussion on a fluorescence detection in microfluidical chips is carried out. A novel, low-cost image sensor - based detection instrumentation co-working with a “clever” software is described. Examples of applications of the novel method co-working with various microfluidical chips are presented.
This paper reports the analysis of drug effects on single breast cancer cells using the droplet-microfluidic devices for the first time. Hundreds of droplets can be generated in less than 5 minutes. Each droplet serves as a nanoliter-volume titer plate. About 50% of the droplets contain a human breast cancer cell MDA-MB-231 and Cytochalasin D drug solution. The drug effects on each cell are monitored...
Our objective is to selectively and non-invasively deposits markers under image guidance for internal tattooing. This study describes the production of ultrasound-inducible particles carrying large payloads of fluorescent markers and the in vivo proof of concept of their remote deposit via focused ultrasound. The particles are double emulsions produced in a microfluidic system, consisting of aqueous...
Herein we discuss two broad approaches for performing high sensitivity optical detection within microfluidic environments. First, we describe recent work in which fluorescence lifetime imaging has been shown to be a sensitive probe of environmental parameters such as pH, viscosity, molecular concentration and temperature. Additionally, we demonstrate how dynamic fluorescence lifetime imaging can be...
In this paper we present the design, simulation and measurement results of a microfluidic optical sensor system for fluorescent sample analysis fabricated by single-layer micro-stereolithographic prototyping in a transparent photoresin. With rapid fabrication and simple packaging processes the device can be manufactured in less than one hour. The analysis efficiency of the sample observation zone...
By combining optical manipulation, fluorescence microscopy and microfluidic devices we have studied the response from different signaling pathways in single Saccharomyces cerevisiae cells upon environmental perturbations. GFP-tagged proteins in the signaling pathway of interest visualized the heterogenic response within a population.
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