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Integration and miniaturization of bio-molecular detection systems into electronic biosensors and lab-on-chip platforms is of great importance. One widely recognized application area for such devices is nucleic acid (DNA and RNA) detection, specifically, nucleic acid amplification testing (NAAT), which relies on enzymatic processes such as polymerase chain reaction (PCR) to increase the copy number...
Presented is a microscope design that employs two-photon non-linear excitation to allow the imaging of the fluorescence from almost any visible fluorophore at resolutions below 30 nm without changing filters or excitation wavelength. The ability of the microscope to image samples at atmospheric pressure, room temperature, and in solution makes it a very promising tool for the biological and materials...
Using the well developed Deep Ocean Raman In Situ Spectrometer (DORISS), we have developed an in situ deep-sea marine sediment pore water probe for geochemical studies. This novel device allows detailed measurement of pore water profiles. Our particular interest is in dissolved CH4, SO4=, H2S, and pH in situ without incurring artifacts due to the degassing that typically occurs with core recovery...
In this paper, we report a membrane filter-based detection system of bacteria using streptavidin functionalized quantum dots (SA-QDs) as a fluorescent probe. The aimed E. coli cells were marked by quantum dots through immuno-recognition, and then the marked bacteria were enriched by a low-fluorescence optical aluminium oxide membrane. A portable system was prepared for measurement of fluorescence...
Optical Coherence Tomography (OCT) is a well known technique for creating non-invasive, high resolution images of biological microstructure. OCT has matured into animportant imaging modality since its invention in 1991. The popularity of the method lies in the numerous advantages that it offers high detection sensitivity allowing fast imaging speed, andhigh spatial resolution. In this paper we propose...
A double-clad photonic crystal fiber was used to improve detection efficiency over a regular single mode fiber in a two-photon fluorescence detection scheme where the dye was excited and the fluorescence was detected back through the same fiber.
Summary form only given. We consider two resonant molecules (or nano-particles) located near the tip of an apertureless near-field scanning optical microscope (NSOM). The tip serves as a probe that allows us to observe the molecules and control the coupling between them. Various models are considered for the nanosized probe tip: a point-like dipole, a small dielectric sphere, and an elongated spheroidal...
Summary form only given. A single fluorescent molecule in an isotropic medium acts as a dipole radiator. In a locally heterogeneous environment the angular emission pattern is modified. Recently Novotny (1996) calculated strongly peaked angular emission when the emitter is brought in close proximity to a strong dielectric-metal gradient. We believe we present the first direct observation of this prediction;...
Summary form only given. With the advent of scanning near-field optical microscopy (SNOM) ultrahigh-resolution optical microscopy has attracted renewed interest. Although after more than a decade the conventional mode of SNOM that uses a subwavelength aperture has bottomed out at a resolution of roughly 50 nm, an unlimited resolution could be expected in optical near-field microscopy. One of the conceptually...
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