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Amyloid β25–35 (Aβ25–35) peptide is a peculiar peptide for its rapid aggregation properties and high neurotoxicity in Alzheimer's disease. Here, the interactions between gold nanoparticles (GNPs) and Aβ25–35 monomers, oligomers and fibrils are explored under different molar ratio, temperature and pH by ultraviolet–visible and circular dichroism spectra, thioflavin T fluorescence assay and transmission...
Gold - poly(dimethylsiloxane) (Au - PDMS) nanocomposite freestanding films were prepared and used to study the binding of Human Serum Albumin (HSA) to polyclonal anti-HSA. A new method of synthesis was developed for sensing application when most of the gold nanoparticles have to be localized in the surface layer. The localized surface plasmon resonance band in gold nanoparticles is used to monitor...
Currently the need for various developmental models in material sciences to develop green technologies has been reemphasized. For biodiversity rich countries like India, low investment and sustainability of biological synthesis adds to its attraction. Plants respond to heavy metal stress by metal complexation process like production of phyochelations or by other metal chelating peptides. In this paper...
A novel optical biosensor with gold surface bound silver nanoparticles has been constructed for detection of bio-molecules like antigen, antibody protein and DNA plasmid, which exhibited distinct optical properties of localized surface plasmon resonance (LSPR). The silver colloidal nanoparticles were prepared by using sodium borohydride reduction of silver nitrate and characterized by using UV-vis...
This paper presents an attempt to use two substrate types silicon and glass for microarray technique, in order to find the best chemistry surface for immobilization of proteins in microarray technology. Different chemical substrate were studied (glass slides and silicon slides coated with gold and poly-L-lysine) printed with BSA and marked with CY3. The slides were printed and after that washed with...
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