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This paper reports a developed DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, and a flatbed scanner to detect the hybridization signal. There was a significant difference in grayscale between the experimental samples and the negative control after 18 min. The detection limit for a DNA sample is 8...
A 10 nm nanogold particle was used to label monoclone CEA antibody to prepare an immunonanogold resonance scattering probe (AuCEAAb) for detection of CEA. In the pH 6.8 Na2HPO4-NaH2PO4 buffer solution and in the presence of PEG-6000, the immunoreaction between CEA and AuCEAAb took place to form the immunonanogold complex that can be removed by centrifugation technique. The supernatant containing AuCEAAb...
This study was designed to develop and evaluate a chitosan-collagen-soybean phosphatidylcholine (SPC) complex film impregnated with mitomycin C (MMC)-PLA- nanoparticles for treatment of hepatocellular carcinoma in mice. MMC-PLA-nanoparticles were prepared by a reverse micelle-solvent evaporation method. PLA-MMC-nanoparticles were dispersed throughout the film. SEM, ATR-FTIR were used to character...
A novel optical biosensor with gold surface bound silver nanoparticles has been constructed for detection of bio-molecules like antigen, antibody protein and DNA plasmid, which exhibited distinct optical properties of localized surface plasmon resonance (LSPR). The silver colloidal nanoparticles were prepared by using sodium borohydride reduction of silver nitrate and characterized by using UV-vis...
An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and surface-enhanced Raman spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (DTNB) that is coimmobilized with biospecific anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-DTNB, which combing both electromagnetic field and chemical enhancement,...
Our lab has used an optical coherence microscope (OCM) to assess both the structure of tissue-engineered corneal constructs and their transparency. Currently, we are not able to resolve cells versus collagen matrix material in the images produced. We would like to distinguish cells in order to determine if they are viable while growing in culture and also if they are significantly contributing to...
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