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Hyperthermia affects certain regulatory proteins, kinases or cyclins, resulting in alternations to the cell cycle and even to apoptosis. Damage to the cell plasma membrane is a key factor in the killing of a cell by hyperthermia. Analysis at the single-cell level is necessary for understanding the fundamental mechanisms of hyperthermia-induced cell death and the generation of thermotolerance in surviving...
Hyperthermia affects certain regulatory proteins, kinases or cyclins, resulting in alternations to the cell cycle and even to apoptosis. Damage to the cell plasma membrane is a key factor in the killing of a cell by hyperthermia. Analysis at the single-cell level is necessary for understanding the fundamental mechanisms of hyperthermia-induced cell death and the generation of thermotolerance in surviving...
The morphological studies of neuron structures are of great interests for biologists. However, manually detecting dendrites structures is very labor intensive, therefore unfeasible in studies that involve a large number of images. In this paper, we propose an automated neuron detection and description method. The proposed method uses ratios of probability maps from random walk sessions to detect initial...
In the last decade new ideas were born about the temporal and spatial dynamics of intercellular calcium waves in astrocytes. In this paper we introduce a new approach to analyze the ways in which astrocytes communicate in cultures. We present a method to describe the spatial propagation of Ca2+ waves in vitro and a technique to compare the activity of different cells in vivo and in vitro under different...
The E.coli expressed carrot antifreeze protein (DcAFP) was purified by preparative HPLC, refolded by dialysis, and checked by analytical HPLC and SDS-PAGE. The gradient-concentrations of DcAFP in 3% glycerol were used to cryopreserve rice single cell by vitrification at -25degC, -75degC and -180degC. The result showed that DcAFP, at optimal concentration of 0.1 mg/ml, can visibly inhibit the liquid...
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