The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
Phylogenetic analysis is a study of evolutionary relationship between organisms or species, and the evolutionary is displayed as a phylogenetic tree. In this paper, the phylogenetic tree was constructed by using distance-based method; UPGMA and the proposed method, MOM estimator. The UPGMA calculates the average of possible pairwise distances to get a new distance in the clustering process. If outliers...
Ebp1 is a member of the PA2G4 family protein and initially isolated as an ErB3 (an epidermal receptor tyrosine kinase) binding protein. Ebp1 inhibits cell growth and repress transcription of E2F-regulated cell cycle genes. Previously, we reported that Ebp1 protein interacted with RNA polymerase subunit PB1 of the influenza virus and disturbed in vitro RNA synthesis by influenza RNA polymerase. Recently...
Influenza virus infection induced various changing of the host cell. At the beginning of influenza virus infection, the transcription from viral genome RNA by RNA-dependent RNA polymerase took place by cleavage of host capped RNA using own endonuclease for priming of mRNA synthesis. After the viral protein NS1 expression, NS1 binds to CPSF lead to inhibit polyA addition of host mRNA (1). Finally the...
HCV (Hepatitis C virus) that the NS3 protease and NS5B RNA-dependent RNA polymerase (RbRp) which the enzymes for virtual replication. HCV plays an important role that to cause the chronic and liver diseases. The computer aided drug design (CADD) that is the new method to design the new molecules as like the drugs from the potent compounds. We took the program of Discovery Studio 2.0 and the scoring...
Influenza virus PB1 protein plays the key role for the assembly and catalytic function of viral RNA polymerase. We showed that Ebp1 interacted with PB1 and interfered both the viral RNA polymerase function in vitro and the virus growth. The Ebp1 expression was induced by influenza virus infection using western blotting and quantitative real time PCR assay. However, the quantitative determination has...
This paper presents a new miniature reverse-transcription polymerase chain-reaction (RT-PCR) system integrating a sample pretreatment device for fast nucleic-acid amplification and diagnosis of viruses and bacteria. In the system, a two-way serpentine-shape (s-shape) pneumatic micropump and a magnetic bioseparator were developed for separation and enrichment of viruses and bacteria. This new bioseparator...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.