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Computational tools are indispensable in the genetic engineering today. This research employs computational analysis on microRNAs which are targeting at the PTEN gene. This study aims to analyze the implication of microRNAs in the expression of PTEN which is leading to tumorigenesis. The analysis of microRNAs sheds light on the regulatory mechanism of PTEN in cancer development.
To investigate the effects of different doses of X-ray on the telomerase in human osteosarcoma (HOS) cells and to provide theoretical evidence for the clinical application of radiotherapy in osteosarcoma. Log phase HOS cells were radiated using 1, 2, 4 and 8 Gy doses of X-ray separately and cell survival was measured using MTT method, telomerase activity by ELISA, and telomerase reverse transcriptase...
RUNX3 is one of the suppressor gene in ovarian cancer, it is not clear whether the expression of RUNX3 is regulated by its promoter methylation or the demethylating agent 5-aza-2'-deoxycytidine. We carried out methylation — specific PCR (MSP) to investigate the methylation status of RUNX3 promoter region in human ovarian cancer cell line 3AO, KOV3 and normal ovarian epithelial cell NOEC. The methylation...
Purposes: constructing prokaryotic vector of Timp3 (tissue inhibitor of metalloproteinase-3). Methords: Total RNA of human placenta tissues were prepared, and the cDNA encoding Timp3 was got via RT-PCR. The gene was constructed into pMD18-T vector using the restriction enzyme of BamHI and XbaI. Results: By agrose gel electrophoresis and DNA sequencing, Timp3 gene was introduced into pMD18-T successly.
In order to explore the effect of paclitaxel (PTX) combined with Survivin siRNA on proliferation and apoptosis in U-87MG cells in vitro, the U-87MG cells were transfected with Survivin siRNA and treated with PTX at the same time. The cell morphological changes were determined by methylene blue solution. Survivin, cyclin D1, c-Myc and CDK4 expression in U-87MG cells was analyzed by RT-PCR and western...
Endoplasmic reticulum stress(ERS)is a kind of sub-cellular pathological process which result from calcium disorderliness and protein improper folding. And endoplasmic reticulum stress induced apoptosis is a novel pathway which different from the mitochondria-initiated intrinsic pathway and the death receptor-triggered extrinsic pathway. Trichosanthin (TCS), a type I ribosome-inactivating protein,...
Purpose: The etiology of idiopathic orbital inflammatory pseudotumors (IOIP) is unknown. Epstein-Barr virus (EBV) infection, which causes significant lymphocyte infiltration, has been proposed to be involved in IOIP. This study tries to validate the relationship between EBV infection and the development of IOIP. Methods: Eleven surgical samples from IOIP patients and four non-IOIP control samples...
This study was comparison of MGMT and ERBB2 expression in human gliomas. RT-PCR method was used to determine the mRNA levels of MGMT and ErbB2 gene in 42 clinical glioma specimens,There was significant concordance between the expression of MGMT and ErbB2 (p < 0.01). MGMT and ErbB are highly remarkably related each other. The results suggest that there may be in relationship among MGMT and ErbB2...
Alternative splicing is the RNA splicing variation mechanism. The exon isoforms lead to numerous different expressions through the alternative splicing. It's imperative to find the carcinogenic gene from the massive quantity of genes and understand the expressed isoforms. In this paper, the statistical procedures are applied on the colon cancer sample data of Affymetrix GeneChip Human Exon 1.0 ST...
The background of this study is Tanshinone was thought to be a potential antitumor drug. In this study, we investigated the apoptosis of hepatoma HepG2 induced by Tanshinone II-A as well as the involved molecules. The inhibitory effects on cell proliferation of Tanshinone II-A were assessed by MTT method. DNA fragmentation was evaluated by agarose gel electrophoresis. Cell cycle and apoptotic rates...
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