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interferen-? of Rongchang porcine was amplifed by RT-PCR with the total RNA extracted from the peripheral blood lymphocytes. The gene was sequenced after cloned into the vector pMD18-T and analyzed with molecular biological software. The results showed that the opening reading frame (ORF) of interferen-? of Rongchang porcine consists of 501 nucleotides, encoding 166 amino acids, the front 23 amino...
We aimed to investigate the expression and location change of ER-a36 in BGC823 when dealt with TAM. Cell growth was examined by WST-1 and assayed by microplate reader. ER-a36 expressions in mRNA and protein were assayed by RT-PCR and Western-blot method and were quantitated. The location of ER-α36 in cell was examined by immunofluorenscence methods. The cell numbers significant decreased at all TAM...
It is reported that circulating miRNAs are potential new biomarkers for diagnostic and other clinical applications. To study the varieties of plasma miRNAs expression, we investigated maternal plasma miRNAs expression profiles using SOLiD sequencing technology. The results showed that the distribution and abundance of specific miRNAs in plasma are varying during physiological pregnancy process. We...
Conditions of the extraction of polysaccharides from protocorms of Dendrobium candidumwere optimized by orthogonal experiment. The results showed that the optimum technology of isolation is to extract about 3 times and 0.5 hours each time in 30 times water at 100°C, the ways to break cell is high-speed homogenate. After 24h, the mRNA expressions of NF-κB was detected by reverse transcription-polymerase...
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