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Observe the influence of solasodine hydrochloride (SBHL) on mouse S180's tumor model's acquired multi-drug resistance by measuring the tumor chemo sensitivity, tumor cell apoptosis and gene expression level of P-glycoprotein(P-gp), Lung Cancer Resistance Protein(LRP) and Topoisomerase II. Methods Establish the Multi-drug Resistance (MDR) mouse S180 tumor model by low dose chemotherapy. Treat the mouse...
Pentobarbital was used to induce the place orientation and space exploration dysfunctions. Morris water maze was used to observe mice's abilities of place orientation and space exploration. Bradford method was used for the determination of the total protein and spectrometry method was used for the detection of acetylcholinesterase (AChE) activity. Extract of Polygala tenuifolia and Poria cocos (EPP)...
The mammalian target of rapamycin (mTOR) is an important controller of cell proliferation. RAD001 (everolimus) is a specific inhibitor of mTOR, can block mTOR signaling pathway. The purposes of this study are to explore the inhibitory effects of RAD001 on Cashmere goat fetal fibroblasts (GFb cells) proliferation. We examined both the effects of RAD001 on cell proliferation and cytostatic effect of...
SET and MYND domain-containing protein 3 (SMYD3) is a novel histone methyltransferase involved in human carcinogenesis. To establish the transgenic cell model of SMYD3 for the further studies on the characteristics and functions of SMYD3, in the present study, the human SMYD3 gene was subcloned into pIRES2-EGFP eukaryotic expressing vector. The recombinant plasmid was transfected into NIH3T3 cells...
Aims: The aim of this study was to investigate the protective effects of FAC in hepatic fibrosis (HF) and the underlined mechanism. Methods: Using KM mice subjected to CCl4-induced HF. At the end of the experiment, the tissues of liver were removed for histopathological examination and SOD, MDA, HYP assays. The expressions of PPARγ were detected by immunohistochemistry. Result: we found that administration...
Using diffuse optical tomography we have imaged early vascular responses to anti-angiogenic treatments in a small animal tumor model. Images acquired from 1 to 7 days after drug administration show measurable changes in hemoglobin concentration. There are observable differences between mice treated with anti-angiogenic drugs and mice that receive a placebo treatment.
A phosphorylatable short peptide(pSP) with the amino acid composition of ??LLLRRRDNEYFYVRRLL?? containing two potentially phosphorylatable tyrosine residues was chemically synthesized. This short peptide was characteristic with the isoelectric point of 10.88 and could be positively charged under physiological condition. The ??DNEYFYV?? motif in the pSP is the substrate of Jak2 kinase which was verified...
To illustrate the possible function of genotoxicity of BBP on DNA, this study took mice livers as experimental materials to detect the levels of DNA-protein crosslinks (DPC), the activity of surperoxide dismutase (SOD), and the contents of malondialdehyde (MDA) with intraperitioneal injection of different doses of BBP for two consecutive 14 days. DPC levels in hepatic cells showed a dose-dependent...
Heroin addiction caused oxidative stress was testified for the first time in mice. After administered with heroin, mice not only showed decreased total antioxidant capacity as well as antioxidant enzymes such as, SOD, CAT and GPx in brain, but also include oxidative damage of DNA, protein and lipid. On the other hand, exogenous antioxidants could restrain the oxidative stress, even alleviate withdrawal...
The purpose of this study is to investigate the relationship between the content of serum interleukin 12 p70 (IL-12 p70) and the growth of sarcoma 180(S180) in mice with S180 after injected with the combined bacterin. After transplanted with S180 in mice, all mice were divided into five groups by completely random design (ie, three groups injected with different single bacterin separately, one group...
As one of important components in traditional Chinese medicines, Musca domestica larvae has been used to cure ecthyma, osteomyelitis, C, malnutritional stagnation and lip boil since the Ming/Qing Danysty. The objective of this study was to evaluate the immunomodulatory effects of the Musca domestica pupae lectin (MPL) on the activity of macrophages and splenocyte. The fraction of buffer insect solution...
To construct a recombinant vaccine alpha-fetoprotein (AFP)-heat shock protein (HSP70) complex, and study its ability to induce specific cytotoxic T lymphocyte (CTL) response and its protective effect against AFP-producing tumor. A recombinant vaccine was constructed by conjugating mouse heat shock protein 70. By way of intracutaneous injection, mice were primed and boosted with recombinant vaccine...
This study was designed to develop and evaluate a chitosan-collagen-soybean phosphatidylcholine (SPC) complex film impregnated with mitomycin C (MMC)-PLA- nanoparticles for treatment of hepatocellular carcinoma in mice. MMC-PLA-nanoparticles were prepared by a reverse micelle-solvent evaporation method. PLA-MMC-nanoparticles were dispersed throughout the film. SEM, ATR-FTIR were used to character...
The protective effect of oxyresveratrol from Ramulus mori against alloxan induced liver damage in mice was examined. In this study, the activities of serum aspartate aminotransferase, serum alanine aminotransferase and superoxide dismutase, catalase and the contents of malonyldialdehyde and reduced glutathione in liver were measured. Mice were divided into five groups as control, model control (only...
To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythrocyte complement receptor 1(CD35) in S180 mice. Red blood cells from mice venous blood are labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Using flow cytometry, we determined the number of CR1. Using microscope, we studied the adherence between erythrocyte...
Antibiotic resistance is a rapidly evolving problem that is not being adequately met by new antimicrobial drugs. Thus, there is a pressing need for effective antibacterial therapies that can be adapted against antibiotic-resistant bacteria. Here, we engineered synthetic bacteriophage to combat antibiotic-resistant bacteria by overexpressing proteins and attacking gene networks which are not directly...
Studies were conducted to establish the biochemical and pharmacological effects of W-2721 after irradiation in the mouse tests for up to 30 days of irradiation. Adult mice were irradiated with 3, 6, and 8 Gy of /sup 60/Co gamma rays with and without pretreatment by WR-2721 at a dose rate of 400 mg/kg body weight. Animals were sacrificed at different postirradiation days to estimate the amount of DNA,...
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