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Nano-systems, often used in biomedical applications for the treatment of a broad category of illnesses, represent one of the nanomedicine approaches recently proposed to target specific drugs only in the region where the disease has been developed. Recently the use of this technique has been proposed with electropulsation, hence taking advantage of the enhanced permeabilization of the cell membrane...
Pulsed electrical fields (PEFs) that weaken the integrity of cellular membranes have been used for a variety of biomedical applications. Recent work suggests that nanosecond PEFs (nsPEFs) might be more beneficial than longer duration PEFs due to their slightly different mechanism of interaction with the cells. The nervous system presents an attractive, yet underutilized, biological target for nsPFE...
Today single cell research is a great interest to analyze cell to cell or cell to environment behavior with their intracellular compounds, where bulk measurement can provide average value. To deliver biomolecules precise and localized way into single living cell with high transfection rate and high cell viability is a challenging and promisible task for biological and therapeutic research. In this...
Despite the significant research in electroporation, high electric field was applied to the whole cells resulted in permeabilizing the membrane of millions of cells without reversibility [1]. To deliver biomolecules through the specific region of the cell membrane with high cell viability and high transfection rate is important for various biological and therapeutic applications.This report presents...
The Electrospinning and Stamp-thru-mold (ESTM) technique, an integrated fabrication process which incorporates the versatility of the electrospinning process for nanofiber fabrication with the non-lithographic patterning ability of the stamp-thru-mold process is introduced. In-situ multilayer stacking of orthogonally aligned nanofibers, ultimately resulting in a nanoporous membrane, has been demonstrated...
Summary form only given. There are a number of studies that show the biological effects of intense pulsed electric fields (PEFs) dependent on the waveform of the pulses such as duration, rise time as well as the number of pulses. PEFs with a pulse duration exceeding 1 us cause the increase in the permeability of cell membrane in other words electroporation. As the duration and rise time of PEF are...
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