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Today, chromatography is the foundation of detachment science and is being utilized as a part of all examination research centers and pharmaceutical enterprises of the world. In these chromatography Ultra Performance Liquid Chromatography (UPLC) is a moderately new procedure giving new conceivable outcomes in fluid chromatography, particularly concerning abatement of time and dissolvable utilization...
A HPLC method for the determination of the content of Isoniazid and Isonicotinic acid in Isoniazid Tablets was established. The determination was performed on SHIM-PACK VP-ODS C18 column. The mobile phase consisted of (40:60) methanol-water (0.2%Docusate Sodium) at a flow rate of 0.8ml·min−1. The UV detection wavelength was set at 261nm and the sample size was 5μL. The linear range of isoniazid and...
Because of Bio-diesel outstanding environmental protection and renewable characteristic, more and more nations are paying attention to it. The content of free glycerin in Biodiesel is one of important targets to evaluate Bio-diesel. In the study, by using high capacity C18 SPE column to remain the major component(long-chain fatty acid alkyl ester) in Bio-diesel in order to eliminate its interfering...
The separation/enrichment of tilmicosin was studied based on the system of aqueous two phase-gas solvent subblation, the method of HPLC was established to determin tilmicosin enriched. The optimal condition of the solvent sublation was obtained: the sublation solvent was n-propanol, the salting-out agent was (NH4)2SO4 with 36% (g/100ml), the pH was 3, N2 flow rate was 20 ml/min, the sublation time...
A method was developed for simultaneously determination of four arsenic speciation including trivalent arsenic (As (III)), pentavalent arsenic (As (V)), monomethylarsinic acid (MMA) and dimethylarsinic acid (DMA) in fruits and vegetables. Arsenic speciation in samples were extracted with methanol/water (50/50, v/v) after samples were dried at 105°C, and then detected by high performance liquid chromatography-hydride...
A fluorescence quantitative PCR method for detection of Di-n-butyl phthalate in water sample has been developed. DBP was extract from water samples using liquid-liquid extraction. The binding-DNA which includes specific primers and pUC19 as template were synthesized by conventional PCR. DBP concentration calculates from standard curve between concentrations of DBP and Ct value by SYBR Green. The lowest...
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