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To establish a sensitive HPLC method for determination of gossypol-acetate (GA) enantiomers in rat plasma. The study involved administration of oral and intravenous to rats. Plasma samples were collected before and during the predetermined time intervals following administration of GA enantiomers, which were analyzed using HPLC method. The method showed a good linear relationship in the calibration...
In this paper, soy isoflavones as raw material, the use of acid hydrolysis of cellulose daidzein, soybean isoflavones by HPLC method and daidzein content of the orthogonal design, the system of the substrate concentration, enzyme concentration, conversion time on the soybean isoflavone daidzein into effect, the best single-factor test verification transformation temperature, pH, to determine the optimal...
A HPLC method for the determination of the content of Isoniazid and Isonicotinic acid in Isoniazid Tablets was established. The determination was performed on SHIM-PACK VP-ODS C18 column. The mobile phase consisted of (40:60) methanol-water (0.2%Docusate Sodium) at a flow rate of 0.8ml·min−1. The UV detection wavelength was set at 261nm and the sample size was 5μL. The linear range of isoniazid and...
The enantiomeric separations of three neonicotinoid insecticides (compounds 1, 2, and 3) were performed on a Chirobiotic V chiral column by high-performance liquid chromatography (HPLC) under a new-polar mobile phase mode, using methanol and ethanol with triethylamine as the basic additive as the mobile phase. Compound 1 obtained no separation under all the mobile phase, while compound 2 and 3 were...
Objective: To develop a HPLC method for determining the contents of schisantherin and deoxyschizandrin in the dispersible tablets of Fructus Schisandrae Sphenanthera. Methods: The samples were extracted with methanol through supersonic wave and determined by HPLC on Hypersil ODS column (250mm × 4.6mm, 5µm) using methanol-water (73∶27, V/V) as a mobile phase. The flow rate was 0.8 mL/min and the detection...
A high-performance liquid chromatography with ultraviolet spectrophotometer detection method for the simultaneous determination of oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC), and doxycycline (DC) residues in chicken liver was developed and validated. Chicken liver was digested using perchloric acid of 5% mass concentration. The chromatographic separation was performed on an...
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