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Interdigitated electrode based electrical biosensors are prominent in biosensor field. The large number of finger electrodes as comb structure gain high sensitivity through electrical measurements. Listeria monocytogenes is a serious food borne pathogen based bacterium that can cause dangerous disease to human, some infection may result in death. In here, biosensor was prepared from 1μm gap Aluminum...
This paper reports a step-wise protocol to immobilize corresponding antibody on gold electrodes for electrochemical measurement of Osteocalcin (Oc), a bone turnover marker, levels in human serum. To our knowledge this is the first such sensor developed for Oc measurement. The magnitude of electrochemical response current was linearly dependent on Oc concentration with an R square of 0.99. The detection...
This research introduces a homemade high throughput surface plasmon resonance imaging electrochemical system which can detect more than 50 spots simultaneously. The SPRI system was constructed in the Kretschmann configuration and the SPR sensor chip served as the working electrode in the EC module. K3Fe(CN)6 solution were tested by the cyclic voltammetry (CV) method and the SPRI method recording more...
This paper describes an electrochemical immunosensor for the detection of target molecules without the use of labeled antibodies. The sensor is based on the combination of a gold electrode covered with a conductive self assembled monolayer (SAM) of mixed thiols and a permeability measurement of an antibody layer anchored on the SAM. The permeability was measured by the square wave voltammetry (SWV)...
In this work we present typical problems and solutions concerning the development of prototyping electrochemical measurement cells for biosensoric applications. The influences of unwanted effects are discussed such as 1) the variation of gold thin film working electrode surface areas due to the technological inaccuracy of the laser ablation electrode patterning; 2) the variation of the working electrode...
In this paper, a high sensitivity carbon nanotubes (CNTs) enhanced piezoelectric diaphragm-based immunosensor array for detecting anti-goat IgGs is reported. Anti-goat IgGs with 6 different concentrations was successfully detected at the same time. The average diameter of CNTs was characterized by AFM, changing from original 58 nm to 82 nm after absorbing goat IgGs. A nearly linear relationship between...
This paper reports a rapid, simple and direct method for the synthesis of gold nanoparticles (AuNPs) in polypyrrole (PPy) film, which provides a biocompatible matrix for the strong adsorption of antibodies. The electrochemical synthesis of AuNPs/PPy composite on gold or carbon electrode occurs within 20 minutes, and does not involve addition of any nanoparticles or reducing agent. This method is compatible...
The scope of this work is to create different biologically active monolayers on gold thin film electrodes applying Self Assembled Monolayer (SAM) technology and to characterize them with electrochemical methods like Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS). Three monolayer structures applying different methods that are used to gain better probe orientation were created...
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