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The study involved 102 Holstein-Friesian cows imported from Sweden, kept on a farm in the West Pomerania Province. Beta-lactoglobulin (LGB) genotypes were determined using PCR (Polymerase Chain Reaction) method according to Medrano andAquilar-Cordova [1990]. It was found that cows with the LGB AA genotype had the highest milk yield in all analysed 305-day lactations. The differences were significant...
The impact of in vitro isolation and molecular characterisation of Neospora caninum as well as sequence analyses was studied. The brain homogenate of a naturally Neospora-infected dairy cow (positive in ELISA and Western blot) was intraperitoneally inoculated into Mongolian gerbils (Meriones unguiculatus). The brain of gerbils on day 60 post-inoculation was homogenized, and, after trypsin-digestion,...
Neospora caninum was isolated from the brain of an apparently healthy calf born to a seropositive cow. The calf was killed 12 hrs after birth and homogenate of its brain was inoculated into Vero cells. Neospora-like tachyzoites were detected 66 days later. The identity of the parasite was confirmed by polymerase chain reaction amplification of N. canium-specific fragments using Np21 and Np6 primers...
Associations were analysed between the bovine growth hormone combined genotypes (GH/AluI-MspI) and milk production traits in a total of 900 Polish Black-and-White (Polish Friesian) cows. PCR-RFLP method was used for genotyping. The following were frequencies of GH/AluI-MspI: 0.463 LL/++, 0.276 LV/++, 0.157 LL/+-, 0.059 LV/+-, 0.025 VV/++, 0.019 LL/-- and 0.001 (only 1 observation) of VV/+-.Significant...
Neospora caninum, an apicomplexan protozoan causes economical losses due to reproductive failure associated with abortion among cattle. The diagnosis of neosporosis is difficult, due to nonspecific clinical signs and there are no parasitological examinations which can help to recognise neosporosis in vivo. Milk of seropositive cows was examined for both, IgG level to antigens of N. caninum and the...
Whole blood samples from 73 animals from a dairy herd infected with BLV were used for detection of proviral DNA by means of PCR technique. At the beginning 24 cows were examined with primer set ZM1/ZM2. Seven out of 13 ELISA-positive animals –were PCR-positive too and 3 were PCR-positive among 11 serologically negative. Due to unsatisfactory results the rest of the samples were tested with a second...
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