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The investigation of proviral DNA by the use of the nested-PCR revealed three Times more positive results than the detection of antibodies by ELISA. Examination of DNA isolated from the whole blood of cattle with the starters ZM2, ZM3 and ZM4, ZM5 of env gene of BLV showed 100% positive results on the farm G and 63.3% on the farm K. All the 15 negative results in ELISA proved to be positive by nested-PCR...
The total usefulness of the nested PCR method in the detection of infections of cattle with BLV has been confirmed. The efficiency of DNA amplification of BLV with the PCR method was studied in 162 cows from 2 farms. These investigations were conducted by the classic (standard) PCR method with ZM2/ZM3 exterior primers and by the nested PCR method with ZM4/ZM5 interior primers. The DNA product amplification...
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