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Multichannel microscopy has emerged as a technique for imaging multiple targets (molecules, protein distributions, etc.) simultaneously. Discovering the relative changes in these targets (i.e. distribution of different proteins) is fundamental for understanding cell structure and function. We describe a new method for quantifying and visualizing relationships between multiple targets, from a set of...
The direct observation and research on the subcellular structure is greatly improved by the advances of digital imaging devices and green fluorescent protein (GFP) labeling techniques at the molecular level. However, the digital imaging devices generate huge amount of data, which makes it an urgent task to develop fast, accurate and automatic data processing technologies for the biomedical research...
Fluorescence imaging techniques are powerful tools in the biological and biomedical sciences, because they are minimally invasive and can be applied to live cells and tissues. It is advantageous to exploit the many properties of fluorescence in imaging experiments.[1–3] We demonstrate a novel experimental arrangement for measurements of intracellular dynamics by simultaneous acquisition of fluorescence...
A micropipette technique was adopted to address the viscoelasticity and its relevance to microtubules and intermediate filaments in rat hepatocytes by treatment with colchicine (Col), acrylamide (Acry) or combination of both. The results showed that: under the concentrations studied, treatment with Acry, Col or combination of both agents resulted in a significant decrease in cell viscoelasticity....
Protein subcellular location is one of the most important determinants of protein function during cellular processes. Changes in protein behavior during the cell cycle are expected to be involved in cellular reprogramming during disease and development, and there is therefore a critical need to understand cell-cycle dependent variation in protein localization which may be related to aberrant pathway...
Protein interactions have great significance in biomedical and bioengineering research. It is a challenge to form an ideal protein surface. Self assembled monolayers (SAMs) is a rather new chemical method which is easily handled to fabricate a well defined protein surface. In this work, we performed alkanethiol monolayers with carboxyl end groups, and mixed alkanethiols monolayers with carboxyl and...
As rapid acquisition of large collections of fluorescence microscopy cell images can be automated, large-scale subcellular localizations of GFP-tagged fusion proteins can be practically accomplished. Semi-supervised learning has the potential of using a large set of unlabeled images for the recognition of subcellular organelle patterns, but the performance still has room for improvement. This paper...
An alternative approach for fabricating a protein array at nanoscale (<100 nm) is suggested with a capability of characterization and/or localization of multiple components on a nanoarray. Basically, fluorescent micro- and nanospheres each conjugated with different proteins are size-dependently self-assembled (SDSA) onto these nanometer wells that were created on the polymethyl methacrylate (PMMA)...
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