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Earlier studies have shown that psoriasis in Japan and Thailand is associated with two different major histocompatibility complex (MHC) haplotypes – those bearing HLA‐Cw6 and those bearing HLA‐Cw1 and HLA‐B46. In an independent case‐control sample from Thailand, we confirmed the association of psoriasis with both haplotypes. No association was seen in Thai HLA‐Cw1 haplotypes lacking HLA‐B46, nor was...
The frequencies of human leukocyte antigen (HLA) class I and class II specificities and haplotypic associations were determined in 1940 unrelated donors from Castilla y León and compared with other Iberian, Mediterranean and European populations. Specificities were determined using polymerase chain reaction reverse sequence‐specific oligonucleotide or polymerase chain reaction sequence‐specific primer...
The aim of this study is to explore human leukocyte antigen (HLA)‐DQ variability in two populations (Cayapas Amerindians and Afro‐Ecuadorians) who live near one another along the Cayapa River and who are exposed to the same environmental stresses, such as infection by Onchocerca volvulus. HLA‐DQA1 and HLA‐DQB1 of 149 unrelated individuals (74 Cayapas and 75 Afro‐Ecuadorians) have been analyzed. HLA...
Approximately five decades ago, alloimmunization to human leukocyte antigens (HLA) and platelet refractoriness were recognized as potentially serious complications of platelet transfusions. The mechanisms that result in stimulating immunity against blood products are still incompletely understood but are related to both the composition of the donor product transfused and the immune status of the recipient...
Human leukocyte antigens (HLA) are important genetic markers of tissue identity and accurately reflect ancestral history. The work reported in this paper provides a detailed description of HLA polymorphism in Polynesian and Maori individuals in relation to other populations. Our study concerns HLA classes I and II antigens in Polynesian (N = 36) and Maori (N = 114) subjects genotyped at two digit...
This study investigated whether killer‐cell immunoglobulin‐like receptor (KIR) genes and human leukocyte antigen (HLA)‐C alleles, receptors and ligands of natural killer cells are associated with the development of human papillomavirus (HPV)‐related cervical disease in Korean women. Blood samples from 132 women with HPV‐related cervical disease and 159 women without HPV infection were collected for...
We describe 2127 new human leukocyte antigen (HLA) class I alleles found in registered stem cell donors. These alleles represent 28.9% of the currently known class I alleles. Comparing new allele sequences to homologous sequences, we found 68.1% nonsynonymous nucleotide substitutions, 28.9% silent mutations and 3.0% nonsense mutations. Many substitutions occurred at positions that have not been known...
We have characterized 372 novel human leukocyte antigen (HLA) class II alleles identified in newly registered stem cell donors, this includes 281 HLA‐DRB1 alleles, 89 HLA‐DQB1 alleles and 2 HLA‐DPB1 alleles. Most novel alleles were single nucleotide variants when compared to their respective most homologous alleles. In 66.4% of all novel alleles non‐synonymous nucleotide variations were identified,...
Human leukocyte antigens (HLA)‐DQA1*01:07 was identified as an HLA‐DQ blank specificity that segregated with the serological HLA‐A2, ‐B7, ‐DR14, ‐DR52 haplotype, which carried DQB1*05:03. The blank specificity of DQA1*01:07‐DQB1*05:03 may be because of lack of reactivity of available typing sera, or disruption of proper assembly of DQ heterodimer. The cDNA sequence of DQA1*01:07 is nearly identical...
We characterized 549 new human leukocyte antigen (HLA) class I and class II alleles found in newly registered stem cell donors as a result of high‐throughput HLA typing. New alleles include 101 HLA‐A, 132 HLA‐B, 105 HLA‐C, 2 HLA‐DRB1, 89 HLA‐DQB1 and 120 HLA‐DPB1 alleles. Mainly, new alleles comprised single nucleotide variations when compared with homologous sequences. We identified nonsynonymous...
Solid‐phase single antigen bead (SAB) assays are standard of care for detection and identification of donor‐specific antibody (DSA) in patients who receive solid organ transplantation (SOT). While several studies have documented the reproducibility and sensitivity of SAB testing for DSA, there are little data available concerning its specificity. This study describes the identification of antibodies...
Traditional DNA‐based typing focuses primarily on interrogating the exons of human leukocyte antigen (HLA) genes that form the antigen recognition domain (ARD). The relevance of mismatching donor and recipient for HLA variation outside the ARD on hematopoietic stem cell transplantation (HSCT) outcomes is unknown. This study was designed to evaluate the frequency of variation outside the ARD in 10...
Background
Sanger‐based DNA sequencing of exons 2+3 of HLA class I alleles from a heterozygote frequently results in two or more alternative genotypes. This study was undertaken to reduce the time and effort required to produce a single high resolution HLA genotype.
Materials and methods
Samples were typed in parallel by Sanger sequencing and oligonucleotide probe hybridization. This workflow,...
Background
Human leukocyte antigen (HLA)‐E is a non‐classical HLA class I molecule that plays a role in both the innate and the adaptive immune response through interaction with receptors on natural killer‐ and T‐cells. The HLA‐E gene is characterized by limited polymorphism compared with the classical HLA loci on chromosome 6. At the start of this study, only 13 variable sites had been identified...
Tuberculosis (TB) occurs as a result of complex interactions between the host immune system and pathogen virulence factors. Human leukocyte antigen (HLA) class II molecules play an important role in the host immune system. However, no study has assessed the association between HLA class II genes and susceptibility to TB caused by specific strains. This study investigated the possible association of...
The lymphocyte crossmatch is currently the only cell‐based compatibility assay performed by histocompatibility laboratories for transplant purposes. While in many transplant programs the complement‐dependent cytotoxicity crossmatch (CDCXM) remains in use, when available, the flow cytometry crossmatch (FCXM) is the method of choice because of its superior sensitivity and specificity. Unfortunately,...
Next generation DNA sequencing is used to determine the HLA‐A, ‐B, ‐C, ‐DRB1, ‐DRB3/4/5, and ‐DQB1 assignments of 1009 unrelated volunteers for the unrelated donor registry in The Netherlands. The analysis characterizes all HLA exons and introns for class I alleles; at least exons 2 to 3 for HLA‐DRB1; and exons 2 to 6 for HLA‐DQB1. Of the distinct alleles present, there are 229 class I and 71 class...
The human leukocyte antigen (HLA) system plays an important role in hematopoietic stem cell transplantation (HSCT) and organ transplantations, immune disorders as well as oncological immunotherapy. However, HLA typing remains a challenging task due to the high level of polymorphism and homology among HLA genes. Based on the high‐throughput next‐generation sequencing data, new HLA typing algorithms...
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