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In fluorescence‐based assays, usually a target molecule is captured using a probe conjugated to a capture surface, and then detected using a second fluorescently labeled probe. One of the most common capture surfaces is a magnetic bead. However, magnetic beads exhibit strong autofluorescence, which often overlaps with the emission of the reporter fluorescent dyes and limits the analytical performance...
In fluorescence‐based assays, the autofluorescence of capture surfaces produces strong background noise. In article number 1803751, Amos Danielli and co‐workers use photobleaching as a method to reduce the autofluorescence of magnetic beads, a commonly used capture surface. The technique yields a 3‐fold improvement in the limit of detection and signal‐to‐noise ratio of a fluorescence‐based immunoassay...
In article number 1805159, David A. Simpson and co‐workers mount magnetic microbeads (grey: helium ion microscopy) on a diamond with a nitrogen vacancy sensing layer, which reveals the resulting magnetic field (blue‐red). This enables rapid magnetic characterization of bead samples on the individual level, as well as an illumination‐free localization method for biocompatible microbeads.
Magnetic microparticles or “beads” are used in a variety of research applications from cell sorting through to optical force traction microscopy. The magnetic properties of such particles can be tailored for specific applications with the uniformity of individual beads critical to their function. However, the majority of magnetic characterization techniques quantify the magnetic properties from large...
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