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In fluorescence‐based assays, usually a target molecule is captured using a probe conjugated to a capture surface, and then detected using a second fluorescently labeled probe. One of the most common capture surfaces is a magnetic bead. However, magnetic beads exhibit strong autofluorescence, which often overlaps with the emission of the reporter fluorescent dyes and limits the analytical performance...
In fluorescence‐based assays, the autofluorescence of capture surfaces produces strong background noise. In article number 1803751, Amos Danielli and co‐workers use photobleaching as a method to reduce the autofluorescence of magnetic beads, a commonly used capture surface. The technique yields a 3‐fold improvement in the limit of detection and signal‐to‐noise ratio of a fluorescence‐based immunoassay...
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