DNA origami has rapidly emerged as a powerful technique to fabricate user‐defined DNA nanostructures. However, the ability to custom‐make patterns on DNA origami template is hampered by the heavy workload and high cost of changing staple DNA (up to several hundred strands per set). Here, a scaffold‐decorated DNA origami method is developed by prescribing the pattern information to the scaffold DNA. For each pixel of an origami, a designed “pixel strand” (P‐strand) is hybridized to the scaffold, strongly preoccupying a specific position and competing with invading staples in a mild origami assembly. To fabricate a new origami pattern, the P‐strand set needs to be replaced with a universal staple set. The yield of thus‐fabricated DNA origami patterns is comparable to a conventional DNA origami with canonical method. One‐pot fabrication of three different nanopatterns in a single test‐tube is further demonstrated. Also, dynamic switch of the pattern is shown. This method provides a generic approach and offers large flexibility for scaling up the nanofabrication with DNA origami by kinetically modulating the reaction pathway of the staples with the scaffold DNA, which represents a novel route in the self‐assembly of complex biomolecular systems.