Introduction: Neuromuscular parameters must be reproducible to examine neuromuscular adaptations in interventional and clinical studies. The reproducibility of neuromuscular parameters for the soleus (SOL), lateral gastrocnemius (LG), and medial gastrocnemius (MG) was assessed over a period of 2 weeks. Methods: Thirteen subjects (27.4 years, 69.5 kg) were tested for numerous electromyographic (e.g., voluntary and electrical evoked EMG) and mechanical (e.g., voluntary activation level) parameters in 3 test sessions. Results: The majority of the data (28 of 34) revealed moderate and substantial reproducibility. Hmax20%/Mmax20% and Vsup/Msup were less reproducible in LG than in MG and SOL. Muscle activity and M‐waves did not differ between muscles. The ICC for the mechanical data was >0.79. Conclusions: The H‐reflex during voluntary contraction of the LG should be considered with caution. Mechanical data on muscle activation level are reproducible. The reproducibility of neuromuscular parameters is sufficient for interventional studies. Muscle Nerve 53: 464–470, 2016