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In this research, we demonstrated a microfluidic chip to pretreat the samples for viral genome assay. The microfluidic chip has the following three functions; (1) Virus purification and enrichment, (2) Viral RNA extraction, and (3) Capture of the targeted virus genome. Hydroxyapatite chromatography, Boom method, and PNA (Peptide Nucleic Acid) were used for the above three functions, respectively....
Using optical tweezers for trapping and infection of influenza virus influenza virus binds selectively G1 phase cell. This result encouraged us to dissolve the difference between G1- and S/G2/M-phage cells. At the beginning, we tried to analyze the sialic acid content of both G1-and S/G2/M-phase cells. Because it had been shown that influenza virus attached to the sialic acid on the cell membrane...
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