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Microsphere-based therapy is used to allow drug release to treat the critical site through the choice and formulation of variations drug-polymer combinations. They are also used to control the release of insoluble drug by the biodegradability of polymer. Chitosan is one of the candidates for the polymer matrix because their biodegradability and biocompatibility. Recently, we prepared and characterized...
Using optical tweezers for trapping and infection of influenza virus influenza virus binds selectively G1 phase cell. This result encouraged us to dissolve the difference between G1- and S/G2/M-phage cells. At the beginning, we tried to analyze the sialic acid content of both G1-and S/G2/M-phase cells. Because it had been shown that influenza virus attached to the sialic acid on the cell membrane...
The introduction of biological macromolecules such as DNA, RNA, and proteins into living cells plays a crucial role in the fundamental analysis of cellular functions and mechanisms in living systems. Therefore, we have been developing an effective platform for the in vitro manipulation and analysis of biological cells at the single-cells. In this paper, we successfully demonstrated a novel intracellular...
Assembling engineered tissues at the mesoscale into an engineered organ has specially attracted our attention since engineered organ usually has a very complex structure and cannot be fabricated simply by using a fixed scaffold. The fabrication of these composite tissues is, however, difficult since many different scaffolds are required. The flexible microsaffold, called “changeable cell culture (C...
Tissues of multicellular organism are formed of a variety of role-different cells. Furthermore, it is known that a certain cell varies in role and size by the maturity degree. The identity of a cell may be distributed roughly by its size. For example, limbus corneae is the border of the cornea and the sclera. Some cells of corneal limbal epithelium have multipotency and high ability to proliferate,...
Cytoskeletal system, which consists of actin, microtubule (MT), and their collaborating proteins and molecular motors, is a native superior micro machine. In order to utilize the performance, attempts to generate various molecular motions in vitro using purified actin, myosin, and their associating proteins, and studies to develop cell-sized liposomes incorporating active actomyosin inside have been...
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