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Gold-coated microcantilevers were used to develop sensitive micromechanical biosensor for detecting mismatch mutations in DNA hybrids. The selective 5'-end immobilization of 25-mer single-stranded (ss) DNA probes to a gold-coated cantilever surface and the specific hybridization of ssDNA targets to the immobilized probe were performed by the direct electric field control. The controlled electric fields...
The reduction in dimensions of biochip arrays is of interest only if the size of the spots containing DNA or proteins is kept uniform. This condition is necessary to properly compare fluorescence signals from the different spots and thus to validate the results. Toward this aim, we propose a fully automated MEMS-based spotter enabling the spatial positioning of the depositing cantilever array and...
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