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The authors describe three-dimensional (3D) motion pictures of dynamic micro objects obtained by parallel phase-shifting digital holographic microscope. The microscope consisted of a Mach-Zehnder interferometer, an inverted magnification optical system, a continuous wave laser, and a polarization imaging camera. An alum crystal sinking in aqueous solution of alum was set for a dynamic micro object...
Fluorescence imaging is one of the important tools for biological field. For three-dimensional (3D) visualization of the fluorescence, we present two approaches. One is the common-path off-axis incoherent digital holography using dual gratings on dual Fresnel lenses. The other method is the image recovery of a defocused 2D fluorescence image by using the defocused distance obtained from the 3D phase...
We describe several techniques for phase-shifting digital holography based on single-pixel detection and microstructured illumination. In particular, we present a high-speed holographic system based on a Mach-Zehnder interferometer that uses a digital micromirror device (DMD) to sample the object with a sequence of phase patterns. The DMD is also used for phase shifting and for wavefront correction...
Multimodal imaging in biology plays an important role. So far the 3D visualization of fluorescence is realized by scanning systems. For faster imaging, the sectioning process intrinsically exhibits limitations in recording speed. We presented common-path off-axis incoherent digital holography using dual gratings on dual Fresnel lenses. Using the proposed method, single-shot, 3D fluorescence imaging...
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