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Fluorescence imaging is one of the important tools for biological field. For three-dimensional (3D) visualization of the fluorescence, we present two approaches. One is the common-path off-axis incoherent digital holography using dual gratings on dual Fresnel lenses. The other method is the image recovery of a defocused 2D fluorescence image by using the defocused distance obtained from the 3D phase...
Multimodal imaging in biology plays an important role. So far the 3D visualization of fluorescence is realized by scanning systems. For faster imaging, the sectioning process intrinsically exhibits limitations in recording speed. We presented common-path off-axis incoherent digital holography using dual gratings on dual Fresnel lenses. Using the proposed method, single-shot, 3D fluorescence imaging...
Digital holography provides a method of the 3-D recording and numerical reconstruction by a simple optical system and a computer. Quantitative measurement and numerical refocusing are major characteristics. So far, many physical parameters such as amplitude, phase, polarization, fluorescence, and spectra can be obtained independently. Recently, multimodal imaging that can obtain simultaneously two...
Multi-modal 3D imaging based on digital holography is reviewed. In the developed system, 3D phase and 2D fluorescence distributions can be measured simultaneously. This characteristic can be used for measurement of live cell activity. We present the preliminary experimental results. One is the observation of microbeads embedded by fluorescence molecules. Second is the observation of plant cell.
Fluorescent imaging and phase contrast imaging are two major techniques for live cell studies. One can obtain those two types of images by switching illumination and filter sets, and this process usually consumes unnecessary time. In order to image dynamical cell behaviors, we have presented a multi- modal digital holographic microscopic method to obtain both quantitative phase images and fluorescent...
Reconstructed position of the optical absorber embedded in a homogeneous scattering medium is evaluated. In the numerical reconstruction, the ratio of the intensity distribution in the scattering medium with an absorber to that in the scattering medium without the absorber is used.
A hybrid digital holographic microscopic system that enables simultaneous detection of 3D phase and fluorescence images is proposed. The proposed microscopic system is designed by combination of a transmission type off-axis digital holographic configuration and a reflection type Fresnel incoherent holographic configuration, aimed for biological application. Owing to the single acquisition, signal...
Fast measurement system of a moving three-dimensional phase object using digital holographic microscopy is presented. In the fabricated system, a three-dimensional phase object is put on a fast movable stage. Under the CW laser illumination, the holograms of the phase object are superimposed. We experimentally investigated the effect of the speed of the movable stage on the reconstructed data.
Phase imaging and fluorescence imaging are two essential methods in biological application. A hybrid digital holographic microscopic system that enables simultaneous detection of 3D phase and 3D fluorescence images is presented. A simulation on the simultaneous imaging shows promising results compared with that of the individual detections. In the experimental results, the acquisition of 3D phase...
Hybrid digital holographic microscopy that combines fluorescence microscopy and digital holographic microscopy into a single system for biological applications is proposed. In the proposed system, a phase image and a fluorescence image can be obtained simultaneously by selecting the different wavelengths of the fluorescent light and the phase measurement. Especially for biological applications,...
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