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Transforming microfluidics based biosensing systems from laboratory research into clinical reality remains an elusive goal despite decades of intensive research. A fundamental obstacle towards the development of fully automated microfluidic diagnostic systems is the lack of an effective strategy for combining multiple pumping., sample preparation, and detection modules into an integrated platform...
Cell stiffness together with other cell functional responses are recognized for their sensitiveness to external stimuli including biochemical and mechanical signals. It was shown that the stiffness over the cell body is heterogeneous, and the subcellular stiffness may correlate with other intracellular activities such as the cytoskeleton reorganization and the contractility dynamics. Here we reported...
The detection of bacterial pathogens has far-reaching impacts in various applications including food safety, water quality monitoring and clinical diagnosis. Owing to this, ongoing efforts have focused on developing biodetectors that are portable, offer rapid measurements and high specificity. Here, we present a microfluidic biosensor capable of fast, multiplexed detection of bacterial pathogens....
The 6th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2012) was held at the Imperial Queen's Park Hotel in Bangkok, Thailand from November 4-7, 2012. IEEE NANOMED is one of the premier annual events organized by the IEEE Nanotechnology Council to bring together physicians, scientists and engineers in the area of Nano/Molecular Medicine and Engineering. The...
The capture of circulating tumor cells (CTCs) in shear flow is studied systematically by driving suspensions of breast cancer cells, with different EpCAM receptor expression levels, through microchannels functionalized with EpCAM antibody ligands at various densities. The dependence of cell capture efficiency on flow rate is established and can be described by an exponential function; a characteristic...
A microfluidic device was designed with two parallel laminar-stream-based surface-modified microchannels to separate motile sperm. This modified sperm sorter provided a new selection that had advances in resolving traditional sorting problems. Microscopic examination showed that sperms with different motility and the results of fluorescence images indicated that the laminar streams are the same as...
This paper reports a mechanostimulation system for studying mechanically induced intercellular calcium signaling in networks of human umbilical vein endothelial cells (HUVECs). By incorporating a capacitive (comb drive) force probe and plasma lithography cell patterning, the roles of biophysical factors, including force, duration, and network architecture, in calcium intercellular communication can...
This paper describes a new method for enhancing the fluorescence signal of a bead-based immunosensor in a digital microfluidic system by dielectrophoresis (DEP) force. A single droplet containing scattered polystyrene microbeads conjugated with fluorescent dye is first moved by electrowetting to 3-phase spiral electrodes. The fluorescent microbeads are then concentrated by DEP force at 145 VRMS over...
The minimum detection limit in isolation of circulating tumor cells (CTCs) from binary mixtures is experimentally explored utilizing microchannel arrays functionalized with EpCAM antibodies. Hence, the tested binary mixtures contained EpCAM-positive and EpCAM-negative cells serving as target and non-target cells, respectively. The ratio of target to non-target cells in the mixtures ranged from 1:1...
This work demonstrates the application of phage display technology for molecular diagnosis utility. We propose a novel phage-displayed peptide which specifically bind to p16INK4a, a cervical cancer biomarker. Whole phage particles were developed as a molecular tracer for ex vivo cells imaging technique. Increase in specific phages binding to p16INK4a overexpressed cells is improved when the cells...
In this study, we propose an integrated micro cell culture system to easily carry out loading, culturing, observation, and collection of biological cells in a microchannel, and quantitatively evaluated the several factors concerning with the cells in the micro-environment. Especially, we evaluated the effect of the substrate material for the cell culture, and the adhesion and the proliferative properties...
We propose a Proton beam writing (PBW) system with an auto scanning program and stage movement in order to entirely solve the narrow scanning and low design problems of PBW technique. We developed the auto scanning program to transform from 3D CAD (Computer Aided Design) data to scanning pattern data which enables to pattern the complex structures more accurate and much faster. On the other hand,...
We developed a system to visualize molecular bindings as colocalization of two Q-dots carried by motor proteins on a microtubule (MT) array with predefined polarities in nanotracks. The MT array was prepared utilizing gliding of MTs in micro/nano structures and a dissociation method of retrogressing MTs. Pairs of molecules for cargo transport was designed and evaluated experimentally. One of molecular...
Human monoclonal antibodies against Rabies virus were selected from non-immunized human scFv library (YAMO-I library) and immunized library (Yamo-Rb library) by using phage display technology. The biopanning was performed for 2–5 rounds by using two types of inactivated rabies vaccines as targets. These are purified vero cell rabies vaccine (PVRV) and purified chick embryo cell vaccine (PCEC). A total...
Embryo culture on a digital microfluidic Chip is presented. The digital microfluidic chip consists of two parallel glass plates with conductive., dielectric., and hydrophobic coatings. ICR mouse embryos are cultivated in the culture media (KSOM + AA) droplets placed in a petri dish for static studies or between the parallel glass plates for dynamic studies. Embryo culture is successful with the conductive...
Over the years, microneedles with varieties of materials and shapes have been fabricated, however, simulation of the penetration of microneedle through the skin are seldom reported. This paper reports on modeling and verification of microneedle-skin interactions. For the first time, a nonlinear hyperelastic model on the micro scale for large deformation is developed to determine several phenomena...
In this study, new magnetic manganese ferrite (MnFe2O4) nanoparticles with a size around 100 nanometer (nm) in diameter were used to improve the performance of an immunoassay for detection of influenza infection. A new microfluidic system was developed to automatically implement the entire detection process. In order to apply these new nanoparticles for influenza detection, the design of the micromixer...
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