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The 3D biomimetic microsystem described in this research integrates various functions of the fallopian tube to serve as an in vitro platform for fertilization and embryonic development. This device mimics the in vivo embryo-endometrial stromal cell monolayer coculture to enhance the group culture of embryos and mechanically culture the embryos in a dynamic manner to enhance their development. The...
This research serves as an in vitro platform for fertilization and embryonic development. Thin polydimethylsiloxane (PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. This device integrates the concentration gradient design and perfusion system for proposed embryo coculture with stromal cells.
A microfluidic device was designed with two parallel laminar-stream-based surface-modified microchannels to separate motile sperm. This modified sperm sorter provided a new selection that had advances in resolving traditional sorting problems. Microscopic examination showed that sperms with different motility and the results of fluorescence images indicated that the laminar streams are the same as...
Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. Thus we fabricated a 3D microfluidic chip which integrates the concentration gradient...
A novel laminar stream-based microfluidic chip that can serve as the high efficient technique for human sperm motility sorting through the use of flow cytometry was proposed in this study. Recently, the selection of good sperms in semen plays an important role in the reproductive medicine. However, the traditional sorting methods are normally expensive and time consuming. This modified microfluidic...
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