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We report contraction-expansion array (CEA) microchannels for high-yield blood plasma separation utilizing differential inertial migration. An abrupt change of the cross-sectional area curves fluid streams and accelerates the flow velocity, which induces Dean flow. Utilizing the balance between inertial lift force and Dean drag force, we were able to separate blood plasma from human whole blood with...
We prepare a microdevice to investigate effects of mechanical stress on permeability of vascular endothelial cells (ECs) toward further progress of nano-medicine such as DDS. An array of cell culture channels as pseudo blood vessels is integrated onto a stretching stimulator (Fig. 1). The stretching stimulator has serially connected pneumatic balloon arrays and generates strain gradation by swelling...
It has been a long-term challenge for the detection of trace explosives for life safety and anti-terrorist. Here, a miniaturized networked sensor for trace explosive detection is presented, which is based on fluorescent quenching of a conjugated polymer. The principle and design of the miniaturized sensors network are described and a serial of experiments are conducted to demonstrate the effective...
We report here a lasing “turn-on” sensory mechanism for highly sensitive vapor detection of amines, utilizing a polyfluorene/palladium tetraphenylporphyrin (PFO/PdTPP) composite film as the sensing medium. A rational ratio of PdTPP could effectively quench the fluorescence of PFO, and the fluorescence recovery occurs after electron donating amine binds to PdTPP, which resulting in a “turn-on” lasing...
We here report a new “turn-on” sensory mechanism for vapor sensing in solid-state based on binding-induced weakening of internal heavy-atom effect. Spin-coating films of 9, 9-diocylfluorene-2, 7-bisthiphene 1 and poly [(9, 9-diocylfuorenyl-2, 7-diyl)-alt (3-hexylthiophene-2, 5-diyl)] 2 were obtained and characterized by absorption and fluorescence spectra. The vapor sensing behaviors of the films...
This paper presents an on-chip, filter-less fluorescence detector using an all-digital, time-resolved imager based on a CMOS single-photon detector array with limited in situ processing. The device comprises an array of pixels capable of detecting single photons integrated with time gating circuitry and a 1-bit memory. The sensor allows on-chip, filter-less fluorescence detection and fluorescence...
Ceramic liposomal nanoparticles, cerasomes, are functionalized with antibodies on their surfaces that act as targeting ligands. This is achieved by using the siloxane network present on the cerasome surface as the foundation for chemical treatment processes previously developed for silicon surfaces. The bio-functionality and physical integrity of the cerasomes are characterized demonstrating successful...
This paper reports a droplet microfluidic, sample-to-answer platform for the detection of disease biomarkers and infectious pathogens using crude biosamples. The platform exploited the dual functionality of silica superparamagnetic particles (SSP) for solid phase extraction of DNA and magnetic actuation. This enabled the integration of sample preparation and genetic analysis within discrete droplets,...
We demonstrate the nanopatterning of multicolored inorganic quantum dot light emitting diodes (QDLED) on silicon for compact multispectral analysis of cancer cells. With well-correlated electroluminescen and photoluminescence spectra, the QDLEDs works as near-field excitation sources to illuminate fluorescently labeled cancer cells. Spectral characteristics of individual cancer cell can be recorded...
We present a portable microendoscope based on a microelectromechanical systems (MEMS) scanner, compound gradient refractive index micro-lenses and a photonics bandgap fiber (PBF). It overcomes the size (2.0 × 1.9 × 1.1 cm3) and weight (less than 3 grams) limitations of conventional two-photon fluorescence microscopy toward freely moving subjects. The microendoscope utilizes a PBF for laser excitation...
This paper reports a planar flow cytometer for the detection of fluorescent particles using evanescent waves based on the liquid total internal reflection (L-TIR). This on-chip flow cytometer is able to form the evanescent waves on the interface between liquids which can be used to detect the fluorescent particles in nanosize with high speeds. The total internal reflection is formed to generate the...
A single-core liquid waveguide is reported to be used in the fluorescence enhancement. The liquid waveguide consists of the high refractive index liquid core and the low refractive index sheath liquid. The diameter of the liquid core can be tuned by sheath flow, and the liquid waveguide can work in different status of the refractive index profiles by the diffusion of the high refractive index solution...
Here we report on a novel, centrifugo-pneumatic valving scheme based on water-dissolvable films. Compared to previous active valving approaches, this valving technique is merely controlled by introducing aqueous solutions to the substrate and allowing for dissolution; therefore lending itself to simple fabrication in microfluidic devices. Furthermore, the ability to tailor films over a range of dissolution...
In this study, we proposed a novel mixing method using alternate pulsed flows from three inlet channels with piezoelectric valveless micropumps. It was experimentally and analytically confirmed that the proposed pulsed mixing of two solutions increased mixing speed with increasing the switching frequency of up to 400 Hz. The mixing time of 3.6 ms for 90 % mixing ratio of two solutions was achieved...
A microfluidic device for protein preconcentration based on the electrokinetic trapping principle is proposed. It comprises a Nafion strip which is simply cut from a commercially available membrane and integrated into a polydimethylsiloxane (PDMS) microfluidic structure using a guiding channel. By applying low voltage (<15 V), the device exhibits a preconcentration factor of >103 of fluorescently...
A portable leukocyte differential count is especially desirable for the emerging needs from bedside and NASA spaceflight healthcare. However, previous efforts to miniaturize the test using microfluidics have shown only a 3-part differential of leukocyte including lymphocyte, monocyte, and neutrophil, whereas the 5-part differential is still lacking. Here we report a complete 5-part differential leukocyte...
We present a novel approach for the fully automated generation of dilution series using a disposable microfluidic cartridge. Fluids are metered, mixed and routed without active valving by defined rotational speed only. As a processing device, a standard lab centrifuge can be used. The dilution ratio can be freely choosen by loading the corresponding volume of sample into the cartridge without the...
A microfluidic device based on hydrodynamic fractionation by size was applied for enrichment of muscle precursor cells obtained from heterogeneous primary cells of muscle tissues. In this paper, the relationship between cell size and quality of regenerative muscles in vitro was focused on. Fractionation of primary cells from rat limbs was demonstrated using a microfluidic device based on hydrodynamics...
We report a parylene-based flexible electroporation chip for in vivo nucleic acid delivery. Benefited from the flexibility of parylene film, our chip fits the natural shape of the electroporated objects, thereby provides a uniform electric field over a large area. In addition, the subject is less likely to be harmed owing to the chip features, including good biocompatibility, less invasive electroporation...
Combination chemotherapies play an important role in clinical cancer treatment. We have previously developed a microfluidic high-throughput drug screening platform and used it for the screening of prostate cancer sensitizing agents. We have further developed the system into a fully automated live cell array platform with uniform cell seeding for better control, and used the platform to investigate...
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